Roth Alejandro D, Liazoghli Dalinda, Perez De Arce Felipe, Colman David R
Laboratorio de Biología Celular de Glias, Departamento de Biología, Facultad de Ciencias, Universidad de Chile, Santiago, Chile,
Montreal Neurological Institute, McGill University, Montreal, Canada.
Biol Res. 2013;46(3):243-9. doi: 10.4067/S0716-97602013000300004.
Myelin sheaths present two distinct domains: compacted myelin spirals and flanking non-compacted cytoplasmic channels, where lipid and protein segregation is established by unknown mechanisms. Septins, a conserved family of membrane and cytoskeletal interacting GTPases, form intracellular diffusion barriers during cell division and neurite extension and are expressed in myelinating cells. Septins, particularly septin 7 (Sept7), the central constituent of septin polymers, are associated with the cytoplasmic channels of myelinating cells. Here we show that Schwann cells deprived of Sept7 fail to wrap around axons from dorsal root ganglion neurons and exhibit disorganization of the actin cytoskeleton. Likewise, Sept7 distribution is dependent on microfilament but not microtubule organization.
紧密排列的髓磷脂螺旋和侧翼的非紧密排列的细胞质通道，脂质和蛋白质的分离通过未知机制得以确立。Septin蛋白是一类保守的与膜和细胞骨架相互作用的GTP酶家族，在细胞分裂和神经突延伸过程中形成细胞内扩散屏障，并在髓鞘形成细胞中表达。Septin蛋白，特别是作为Septin聚合物核心成分的Septin 7，与髓鞘形成细胞的细胞质通道相关。在此我们表明，缺失Sept7的施万细胞无法包裹背根神经节神经元的轴突，并且肌动蛋白细胞骨架出现紊乱。同样，Sept7的分布依赖于微丝而不是微管的组织。
