CD14 基因变异及其对儿童喘鸣、特应性和哮喘的重要性。
CD14 gene variants and their importance for childhood croup, atopy, and asthma.
机构信息
Canadian Centre for Health and Safety in Agriculture, University of Saskatchewan, P.O. BOX 23, 104 Clinic Place, Saskatoon, SK, Canada S7N 5E5 ; College of Nursing, University of Saskatchewan, University of Saskatchewan, 104 Clinic Place, Saskatoon, SK, Canada S7N 5E5.
Canadian Centre for Health and Safety in Agriculture, University of Saskatchewan, P.O. BOX 23, 104 Clinic Place, Saskatoon, SK, Canada S7N 5E5.
出版信息
Dis Markers. 2013;35(6):765-71. doi: 10.1155/2013/434920. Epub 2013 Nov 21.
BACKGROUND
The CD14 gene has an important role in the detection of inflammatory provoking pathogens and in the ensuing signaling of the innate immune response. We assessed the role of CD14 C-159T, G-1359T in the expression of asthma, croup, and allergy in Canadian school children of ages 6 to 14 years.
METHODS
Children attending schools in a rural community participated in a cross-sectional survey of respiratory health. Following consent, we conducted clinical assessments to collect buccal swabs for genotyping and perform skin prick testing (SPT) to determine atopic status. Genotyping and SPT results were available for 533 and 499 children, respectively. Separate multivariable analyses that included both polymorphisms were conducted for each phenotype.
RESULTS
The prevalence of asthma, allergy, and croup was 18.6%, 22.4%, and 6.6%, respectively. Children with the T/T variant of CD14 G-1359T were more likely to have physician diagnosed asthma (26.8%). Children with C/C variant of CD14 C-159T had a significantly lower prevalence of croup (2.6%). Haplotype analyses of the two CD14 polymorphisms showed that individuals with the T|T haplotype combination were significantly more likely to have asthma (P = 0.014).
CONCLUSIONS
In this study, CD14 variants are important for the expression of croup and asthma but not atopy.
背景
CD14 基因在检测炎症病原体和固有免疫反应的信号转导中起着重要作用。我们评估了 CD14 C-159T、G-1359T 基因多态性在加拿大 6 至 14 岁学龄儿童哮喘、喘鸣和过敏表型中的作用。
方法
参加农村社区学校的儿童参与了一项呼吸道健康的横断面调查。征得同意后，我们进行了临床评估，采集口腔拭子进行基因分型，并进行皮肤点刺试验（SPT）以确定特应性状态。分别有 533 名和 499 名儿童的基因分型和 SPT 结果可用。对于每种表型，我们分别进行了包括两种多态性的多变量分析。
结果
哮喘、过敏和喘鸣的患病率分别为 18.6%、22.4%和 6.6%。CD14 G-1359T 中 T/T 变异型的儿童更有可能被诊断为哮喘（26.8%）。CD14 C-159T 中 C/C 变异型的儿童喘鸣的患病率明显较低（2.6%）。两种 CD14 多态性的单体型分析表明，T|T 单体型组合的个体更有可能患哮喘（P=0.014）。
结论
在这项研究中，CD14 变体对喘鸣和哮喘的表达很重要，但对过敏没有影响。
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