Kihara F, Tsuji Y, Miura M, Ishibashi S, Ide T
Mech Ageing Dev. 1986 Dec;37(2):103-17. doi: 10.1016/0047-6374(86)90069-2.
When senescent human diploid cells, TIG-1, were stimulated with serum at the end of their proliferative life span, such biochemical events as uptakes of 2-deoxyglucose and uridine, and expression of c-myc, were enhanced. However, RNA synthesis, polyamine accumulation, thymidine uptake and DNA synthesis were not enhanced at all. Protein synthesis increased only moderately as compared with that observed in younger cells. These results indicated that the events in prereplicative phase known to be independent on protein synthesis are induced in senescent cells after the stimulation with serum, whereas those required protein synthesis failed to increase to the same extent as seen in young cells.
当衰老的人二倍体细胞TIG-1在其增殖寿命结束时用血清刺激时，会增强2-脱氧葡萄糖和尿苷的摄取以及c-myc的表达等生化事件。然而，RNA合成、多胺积累、胸苷摄取和DNA合成根本没有增强。与年轻细胞相比，蛋白质合成仅适度增加。这些结果表明，已知与蛋白质合成无关的复制前期事件在衰老细胞经血清刺激后被诱导，而那些需要蛋白质合成的事件未能像在年轻细胞中那样增加到相同程度。
