用苯扎贝特诱导培养的3T3-L1前脂肪细胞向脂肪细胞转化。

Adipocyte conversion of cultured 3T3-L1 preadipocytes by bezafibrate.

作者信息

Brandes R, Hertz R, Arad R, Naishtat S, Weil S, Bar-Tana J

出版信息

Life Sci. 1987 Mar 9;40(10):935-41. doi: 10.1016/0024-3205(87)90312-2.

Abstract

Transient exposure of cultured 3T3-L1 preadipocytes to hypolipidemic fibrate drugs results in extensive adipocyte conversion. Adipocyte conversion in culture was characterized by an increase in neutral lipids content and in adipocyte marker enzymes like hormone-sensitive lipase and glycerol-3-phosphate dehydrogenase. Adipocyte conversion in culture was also accompanied by induction of cyanide-insensitive peroxisomal palmitoyl-CoA oxidation. The conversion pattern exerted by fibrate drugs in 3T3-L1 cells was similar to that reported previously for primary cultured epididymal preadipocytes (R. Brandes, R. Arad and J. Bar-Tana, Biochim. Biophys. Acta, 877, 314-321 (1986)), and seems to refute clonal selection in the conversion sequel initiated by fibrate drugs in primary cultured preadipocytes.

摘要

将培养的3T3-L1前脂肪细胞短暂暴露于降血脂的贝特类药物会导致大量脂肪细胞转化。培养过程中的脂肪细胞转化表现为中性脂质含量增加，以及激素敏感性脂肪酶和甘油-3-磷酸脱氢酶等脂肪细胞标记酶增加。培养过程中的脂肪细胞转化还伴随着对氰化物不敏感的过氧化物酶体棕榈酰辅酶A氧化的诱导。贝特类药物在3T3-L1细胞中产生的转化模式与先前报道的原代培养附睾前脂肪细胞的转化模式相似（R. 布兰德斯、R. 阿拉德和J. 巴尔-塔纳，《生物化学与生物物理学报》，877, 314 - 321 (1986)），并且似乎驳斥了贝特类药物在原代培养前脂肪细胞引发的转化后续过程中的克隆选择。