Brandes R, Arad R, Bar-Tana J
Biochim Biophys Acta. 1986 Jun 27;877(2):314-21. doi: 10.1016/0005-2760(86)90309-7.
Cultured rat epididymal preadipocytes exposed for 24-72 h to either bezafibrate or clofibrate added to the culture medium were extensively converted to fat-loaded adipocytes. Adipocyte conversion increased during the first 5-7 days following plating, reaching a level of 100% and 60% conversion with bezafibrate and clofibrate, respectively, as compared to 10% conversion in their absence. Adipocyte conversion in culture was a saturable function of the hypolipidemic effectors and was associated with an increase in the incorporation rate of exogenous palmitate into triacylglycerols, in glycerol-3-phosphate dehydrogenase and hormone-sensitive lipase activities but not in lipoprotein lipase activity. Adipocyte conversion by hypolipidemic drugs was much more prominent than that exerted by dibutyryl cAMP, and the relative conversion efficiency of the two fibrate drugs did not correlate with their respective cAMP content of the culture. Hence, hypolipidemic drugs and dibutyryl cAMP appear to act independently in initiating adipose conversion in primary epididymal preadipocytes.
将苯扎贝特或氯贝丁酯添加到培养基中，培养24 - 72小时的大鼠附睾前脂肪细胞会大量转化为充满脂肪的脂肪细胞。接种后的前5 - 7天内，脂肪细胞转化率增加，与未添加药物时10%的转化率相比，添加苯扎贝特和氯贝丁酯后的转化率分别达到100%和60%。培养过程中的脂肪细胞转化是降血脂效应物的饱和功能，并且与外源性棕榈酸掺入三酰甘油的速率增加、甘油-3-磷酸脱氢酶和激素敏感性脂肪酶活性增加相关，但与脂蛋白脂肪酶活性无关。降血脂药物引起的脂肪细胞转化比二丁酰环磷腺苷更显著，两种贝特类药物的相对转化效率与其在培养物中的各自环磷腺苷含量无关。因此，降血脂药物和二丁酰环磷腺苷在启动原代附睾前脂肪细胞的脂肪转化过程中似乎独立起作用。
