Krivi G G, Rowold E
Mol Immunol. 1986 Dec;23(12):1381-9. doi: 10.1016/0161-5890(86)90024-6.
Three distinct antigenic regions of bovine somatotropin (bST) were identified on the basis of the ability of a set of monoclonal antibodies to bind to proteolytic fragments and deletion variants of recombinant bST (rbST) in Western blot analyses. One of the regions is further subdivided into two epitopes on the basis of the cross-reaction of somatotropins from several species with the same set of antibodies in solid-phase RIA. The RIA and Western blot results suggest that amino acids 134-150, 181-190 and the amino terminus may be involved in the binding specificity of antibodies to the bovine somatotropin molecule. The total of four antigenic regions on the bST molecule parallels results described for human somatotropin. Labeled antibody competition tests were used to show that the epitope involving amino acids 134-150 is spatially separated from the other three epitopes.
基于一组单克隆抗体在蛋白质印迹分析中与重组牛生长激素（rbST）的蛋白水解片段和缺失变体结合的能力，确定了牛生长激素（bST）的三个不同抗原区域。根据来自几个物种的生长激素在固相放射免疫分析中与同一组抗体的交叉反应，其中一个区域进一步细分为两个表位。放射免疫分析和蛋白质印迹结果表明，氨基酸134 - 150、181 - 190和氨基末端可能参与抗体与牛生长激素分子的结合特异性。bST分子上总共四个抗原区域与人类生长激素所描述的结果相似。使用标记抗体竞争试验表明，涉及氨基酸134 - 150的表位在空间上与其他三个表位分离。
