Krivi G G, Rowold E
Hybridoma. 1984 Summer;3(2):151-62. doi: 10.1089/hyb.1984.3.151.
Twenty-nine stable hybridoma cell lines secreting monoclonal antibodies to bovine somatotropin (bST) have been produced and characterized. Five of the monoclonal antibodies bind porcine and human somatotropins as well as bST. One of these antibodies was used as a reagent in immunoadsorbent chromatography of recombinant bST or pituitary bST from cell extracts. Following chromatography, the bST preparations retained activity in a rabbit liver radioreceptor assay and in a radioimmunoassay. The immunoadsorbent reagent bound human and porcine somatotropins as well as bovine.
已制备并鉴定了29个分泌抗牛生长激素（bST）单克隆抗体的稳定杂交瘤细胞系。其中5种单克隆抗体可与猪生长激素、人生长激素以及牛生长激素结合。其中一种抗体被用作从细胞提取物中免疫吸附层析重组bST或垂体bST的试剂。层析后，bST制剂在兔肝放射受体测定和放射免疫测定中保持活性。该免疫吸附试剂可与人、猪生长激素以及牛生长激素结合。
