Winger C M, Canning E U, Culverhouse J D
Parasitology. 1987 Feb;94 ( Pt 1):17-27. doi: 10.1017/s0031182000053415.
A monoclonal antibody-producing hybridoma cell line has been raised against merozoites of Babesia divergens. This antibody is strongly reactive against merozoites in an enzyme-linked immunosorbent assay (ELISA) and is also positive in an indirect fluorescent antibody test (IFAT) on preparations of live merozoites. The antibody recognizes an antigen, of molecular weight (Mr) 50-60 K by Western blot analysis on SDS-polyacrylamide gel electrophoresis (SDS-PAGE) gels. Merozoite neutralization assays show that the antibody significantly inhibits merozoite invasion of bovine erythrocytes.
已培育出一种针对分歧巴贝斯虫裂殖子产生单克隆抗体的杂交瘤细胞系。该抗体在酶联免疫吸附测定（ELISA）中对裂殖子具有强烈反应性，并且在活裂殖子制剂的间接荧光抗体试验（IFAT）中也呈阳性。通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）凝胶上的蛋白质印迹分析，该抗体识别一种分子量（Mr）为50 - 60K的抗原。裂殖子中和试验表明，该抗体显著抑制裂殖子对牛红细胞的侵袭。
