Gray J S, Kaye B
Faculty of Agriculture, University College, Belfield, Dublin, Ireland.
Vet Parasitol. 1991 Aug;39(3-4):215-24. doi: 10.1016/0304-4017(91)90038-w.
Babesia divergens antigen obtained from bovine and gerbil erythrocytes was compared for the diagnosis of bovine babesiosis by the indirect immunofluorescence antibody test (IFAT) and the enzyme-linked immunosorbent assay (ELISA). Using a range of sera from the field and from experimental cattle, it was found that antigen from the two sources did not differ significantly in sensitivity or specificity. Antigen from gerbils may, therefore, be used for serological surveys of bovine babesiosis caused by B. divergens. The IFAT and ELISA were also compared and it was concluded that they are equally effective in the detection of positive samples. For small-scale surveys, the IFAT using gerbil-derived antigen is adequate, but for large surveys the ELISA is preferable.
通过间接免疫荧光抗体试验（IFAT）和酶联免疫吸附测定（ELISA），比较了从牛和沙鼠红细胞中获得的分歧巴贝斯虫抗原用于诊断牛巴贝斯虫病的效果。使用一系列来自现场和实验牛的血清，发现来自这两种来源的抗原在敏感性或特异性上没有显著差异。因此，沙鼠来源的抗原可用于对由分歧巴贝斯虫引起的牛巴贝斯虫病进行血清学调查。还对IFAT和ELISA进行了比较，得出它们在检测阳性样本方面同样有效的结论。对于小规模调查，使用沙鼠来源抗原的IFAT就足够了，但对于大规模调查，ELISA更可取。
