Kumar Amod, Vn Muhasin Asaf, Raut Ashwin Ashok, Sood Richa, Mishra Anamika
Division of Animal Genetics, Indian Veterinary Research Institute, Bareilly, India.
High Security Animal Disease Laboratory, Bhopal, India.
Bioinform Biol Insights. 2014 Jun 12;8:135-45. doi: 10.4137/BBI.S14631. eCollection 2014.
Highly pathogenic Avian influenza (HPAI) is a notifiable viral disease caused by avian influenza type A viruses of the Orthomyxoviridae family. Type A influenza genome consists of eight segments of negative-sense RNA. RNA segment 2 encodes three proteins, PB1, PB1-F2, and N40, which are translated from the same mRNA by ribosomal leaky scanning and reinitiation. Since these proteins are critical for viral replication and pathogenesis, targeting their expression can be one of the approaches to control and resist HPAI. MicroRNAs are short noncoding RNAs that regulate a variety of biological processes such as cell growth, tissue differentiation, apoptosis, and viral infection. In this study, a set of 300 miRNAs expressed in chicken lungs were screened against the HPAI virus (H5N1) segment 2 with different screening parameter like thermodynamic stability of heteroduplex, seed sequence complementarity, conserved target sequence, and target-site accessibility for identifying miRNAs that can potentially target the transcript of segment 2 of H5N1. Chicken miRNAs gga-mir-133c, gga-mir-1710, and gga-mir-146c* are predicted to target the expression of PB1, PB1-F2, and N40 proteins. This indicates that chicken has genetic potential to resist/tolerate H5N1 infection and these can be suitably exploited in designing strategies for control of avian influenza in chicken.
高致病性禽流感（HPAI）是一种须通报的病毒性疾病，由正黏病毒科的甲型禽流感病毒引起。甲型流感病毒基因组由8个负链RNA片段组成。RNA片段2编码三种蛋白质，PB1、PB1-F2和N40，它们通过核糖体移码扫描和重新起始从同一mRNA翻译而来。由于这些蛋白质对病毒复制和发病机制至关重要，靶向它们的表达可能是控制和抵抗高致病性禽流感的方法之一。微小RNA是短的非编码RNA，可调节多种生物学过程，如细胞生长、组织分化、细胞凋亡和病毒感染。在本研究中，针对高致病性禽流感病毒（H5N1）片段2，筛选了一组在鸡肺中表达的300种微小RNA，采用了不同的筛选参数，如异源双链体的热力学稳定性、种子序列互补性、保守靶序列和靶位点可及性，以鉴定可能靶向H5N1片段2转录本的微小RNA。预测鸡微小RNA gga-mir-133c、gga-mir-1710和gga-mir-146c*可靶向PB1、PB1-F2和N40蛋白的表达。这表明鸡具有抵抗/耐受H5N1感染的遗传潜力，可在设计鸡禽流感防控策略时加以适当利用。
