Lin Peng, Wang Jianke, Song Shanshan, Cheng Yuening, Yi Li, Cheng Shipeng, Wang Zhenjun
Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Changchun, China.
Hebei Veterinary Biotechnology Innovation Center, College of Veterinary Medicine, Hebei Agricultural University, Baoding, China.
Front Microbiol. 2022 Mar 9;13:839320. doi: 10.3389/fmicb.2022.839320. eCollection 2022.
Although mink enteritis virus (MEV) is an acute, virulent, and highly contagious pathogen in minks, there is currently a lack of a quick diagnostic method. By conjugating colloidal gold nanoparticles with the MEV-specific monoclonal antibody, monoclonal antibody (MAb) 14, we developed a single-step competitive immunochromatographic strip (ICS) assay for simple determination of MEV. The optimal concentrations of the colloidal gold-coupled MAb 14 (coating antibody), the capture protein (MEV VP2 protein), and the goat anti-mouse antibody were 1.0, 0.8, and 1.0 mg/ml, respectively. The limit of detection was approximately 512 hemagglutination units/100 μl of MEV B strain. Other common viruses of mink were tested to evaluate the specificity of the ICS, and the results showed no cross-reactivity for other pathogens. In comparison with the Anigen Rapid canine parvovirus (CPV) Ag Test Kit (BioNote, Korea) in testing 289 samples, the percentage of agreement and relative sensitivity and specificity of the MEV ICS assay were 94.1, 93.2, and 97.1%, respectively. The ICS test was found to be a sufficiently sensitive and specific detection method for the convenient and rapid detection of MEV.
尽管水貂肠炎病毒（MEV）是水貂的一种急性、烈性且高度传染性的病原体，但目前缺乏快速诊断方法。通过将胶体金纳米颗粒与MEV特异性单克隆抗体14结合，我们开发了一种单步竞争性免疫层析试纸条（ICS）检测方法，用于简单检测MEV。胶体金偶联单克隆抗体14（包被抗体）、捕获蛋白（MEV VP2蛋白）和山羊抗小鼠抗体的最佳浓度分别为1.0、0.8和1.0mg/ml。检测限约为512血凝单位/100μl MEV B株。检测了水貂的其他常见病毒以评估ICS的特异性，结果表明对其他病原体无交叉反应。在检测289份样本时，与Anigen Rapid犬细小病毒（CPV）抗原检测试剂盒（韩国BioNote公司）相比，MEV ICS检测方法的一致性百分比、相对灵敏度和特异性分别为94.1%、93.2%和97.1%。发现ICS检测是一种用于方便、快速检测MEV的足够灵敏和特异的检测方法。
