Chang D D, Clayton D A
Science. 1987 Mar 6;235(4793):1178-84. doi: 10.1126/science.2434997.
Ribonuclease mitochondrial RNA processing, a site-specific endoribonuclease involved in primer RNA metabolism in mammalian mitochondria, requires an RNA component for its activity. On the basis of copurification and selective inactivation with complementary oligonucleotides, a 135-nucleotide RNA species, not encoded in the mitochondrial genome, is identified as the RNA moiety of the endoribonuclease. This finding implies transport of a nucleus-encoded RNA, essential for organelle DNA replication, to the mitochondrial matrix.
核糖核酸酶线粒体RNA加工是一种参与哺乳动物线粒体引物RNA代谢的位点特异性核糖核酸内切酶，其活性需要一种RNA成分。基于共纯化和用互补寡核苷酸进行选择性失活，一种135个核苷酸的RNA物种（未在线粒体基因组中编码）被鉴定为该核糖核酸内切酶的RNA部分。这一发现意味着一种对细胞器DNA复制至关重要的核编码RNA被转运到线粒体基质中。
