可注射凝胶移植物用于骨缺损修复。
Injectable gel graft for bone defect repair.
机构信息
Division of Plastic & Reconstructive Surgery, Department of Surgery, Keck Medical School, University of Southern California, 1333 San Pablo St, BMT 302A, Los Angeles, CA 90089-9112, USA.
出版信息
Regen Med. 2014 Jan;9(1):41-51. doi: 10.2217/rme.13.76.
AIM
To examine the performance of an injectable gel graft made of transglutaminase (Tg)-crosslinked gelatin gel with BMP-2 (BMP-2-Tg-Gel) for bone defect repair in animal models.
MATERIALS & METHODS: BMP-2 mixed with gelatin gel was crosslinked using Tg. The release of tethered BMP-2 through autocrine and paracrine pathways was demonstrated by using C2C12 and NIH 3T3 cells, respectively. BMP-2-Tg-Gel was injected into the induced cranial defect site. After 14 days, the sample was removed for x-ray imaging and histological evaluation.
RESULTS
Our in vivo results demonstrated that the injectable Tg-Gel with its osteoconductivity and controllable BMP-2 activity induced bone formation in our rat models when tethered with BMP-2.
CONCLUSION
Tg-Gel as an injectable functional bone graft may enable the use of minimally invasive surgical procedures to treat irregular-shaped bone defects. Furthermore, this novel approach is capable of incorporating and controlling the release of therapeutic agents that may advance the science of tissue regeneration.
目的
研究一种由转谷氨酰胺酶（Tg）交联明胶凝胶制成的可注射凝胶移植物（BMP-2-Tg-Gel）在动物模型中用于骨缺损修复的性能。
材料与方法
将 BMP-2 与明胶凝胶混合，然后使用 Tg 进行交联。通过 C2C12 和 NIH 3T3 细胞分别证明了通过自分泌和旁分泌途径释放固定的 BMP-2。将 BMP-2-Tg-Gel 注入诱导的颅缺损部位。14 天后，取出样本进行 X 射线成像和组织学评估。
结果
我们的体内结果表明，当与 BMP-2 结合时，具有骨诱导性和可控制的 BMP-2 活性的可注射 Tg-Gel 在我们的大鼠模型中诱导了骨形成。
结论
作为一种可注射的功能性骨移植物，Tg-Gel 可能能够采用微创外科手术来治疗不规则形状的骨缺损。此外，这种新方法能够结合和控制治疗剂的释放，从而推进组织再生科学的发展。
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