Tron V, Wright J L, Churg A
Arch Pathol Lab Med. 1987 Mar;111(3):291-3.
Immunohistologic markers have been of considerable value in differentiating malignant mesothelioma from adenocarcinoma. Recently, staining for milk-fat globule (MFG) protein has been suggested as a useful diagnostic test for this separation, but subsequent reports have been conflicting, with some authors finding clearcut differences, while others showed similar marking of both tumor types. To examine this technique further, we studied lung carcinomas and mesotheliomas with commercially available anti-MFG, and compared these results with those obtained with anticarcinoembryonic antigen (CEA), a commonly used immunomarker of carcinoma. We found that carcinomas showed strong cytoplasmic staining for MFG and CEA; however, a greater percentage of carcinomas were more strongly positive for CEA than for MFG. Mesotheliomas did not, for the most part, stain strongly with either antibody. In addition, carcinomas from different hospitals stained differently for MFG, but not for CEA. We conclude that although strong cytoplasmic staining for MFG is a reasonably reliable indicator of carcinoma, CEA staining provides a better separation and is considerably easier to interpret in lung cancer specimens.
免疫组织学标志物在鉴别恶性间皮瘤与腺癌方面具有重要价值。最近，有人提出检测乳脂肪球（MFG）蛋白染色可作为区分二者的有用诊断方法，但后续报告结果不一，一些作者发现有明显差异，而另一些人则显示两种肿瘤类型的标记相似。为进一步研究该技术，我们使用市售抗MFG抗体研究了肺癌和间皮瘤，并将这些结果与使用癌胚抗原（CEA）（一种常用的癌免疫标志物）获得的结果进行比较。我们发现，癌组织对MFG和CEA均显示强细胞质染色；然而，更大比例的癌组织对CEA的阳性反应强于对MFG的阳性反应。间皮瘤在大多数情况下，两种抗体染色均不强。此外，来自不同医院的癌组织对MFG的染色不同，但对CEA的染色无差异。我们得出结论，尽管MFG的强细胞质染色是癌的合理可靠指标，但CEA染色能更好地区分，并且在肺癌标本中更易于解释。
