Faure M, Mauduit G, Schmitt D, Kanitakis J, Demidem A, Thivolet J
Br J Dermatol. 1987 Feb;116(2):161-70. doi: 10.1111/j.1365-2133.1987.tb05807.x.
Human keratinocytes from small skin specimens were grown on mouse 3T3 cell feeder layers into epidermal sheets free from Langerhans cells and MHC class II antigen. These were found to be suitable for the permanent coverage of wounds when used as autografts or allografts. We report here the ultrastructural differentiation of this cultured epidermis after grafting onto autologous or allogeneic recipients. The cultured epidermis was a thin but multilayered Malpighian epithelium composed of keratinocytes at different stages of differentiation. The dermo-epidermal basement membrane was newly synthesized during the first few days following transplantation onto de-epidermized wounds. The analysis of keratins and examination of various keratinocyte membrane antigens by immunofluorescence indicated that full terminal epithelial differentiation was only achieved after in vivo transplantation of the cultured epidermis. Langerhans cells, absent in cultures, progressively colonized the grafts, while melanocytes, not detectable in sections of the cultures, were identified among the keratinocytes 2 weeks after grafting.
从小片皮肤标本中获取的人角质形成细胞，在小鼠3T3细胞饲养层上生长，形成不含朗格汉斯细胞和MHC II类抗原的表皮片。当用作自体移植或异体移植时，发现这些表皮片适合永久覆盖伤口。我们在此报告将这种培养的表皮移植到自体或异体受体后其超微结构的分化情况。培养的表皮是一层薄但多层的马尔皮基上皮，由处于不同分化阶段的角质形成细胞组成。在移植到去表皮伤口后的头几天内，新合成了真皮 - 表皮基底膜。对角蛋白的分析以及通过免疫荧光对各种角质形成细胞膜抗原的检测表明，培养的表皮只有在体内移植后才实现完全的终末上皮分化。培养物中不存在的朗格汉斯细胞逐渐在移植物中定植，而在培养物切片中无法检测到的黑素细胞，在移植后2周在角质形成细胞中被识别出来。
