Myers S R, Navsaria H A, Brain A N, Purkis P E, Leigh I M
Department of Experimental Dermatology, Royal London Hospital.
J Clin Pathol. 1995 Dec;48(12):1087-92. doi: 10.1136/jcp.48.12.1087.
To establish the structural changes that occur in deep surgical wounds engrafted with allogeneic sheets, their time course and inter-relation.
Deep surgical wounds following shave excision of tattoos (down to deep dermis/subcutaneous fat) were treated with sheets of sex mismatched allogeneic keratinocytes in 19 patients and then biopsied weekly until wound healing was complete. More superficial surgical wounds--that is, 20 standard skin graft donor sites, were biopsied at seven to 10 days (all healed) following application of keratinocyte allografts. All biopsy specimens were examined with a large panel of monoclonal antibodies to keratins, envelope proteins, basement membrane components, and to extracellular matrix components.
The hyperproliferative keratin pair K6/16 was expressed in all wounds, for up to six weeks in keratinocyte grafted deep wounds, and up to six months in split thickness skin grafted wounds.
Keratins 6 and 16 have not been detected in normal skin, although the relevant mRNA has. This raises the possibility of regulation at a post-transcriptional level allowing a rapid response to injury with cytoskeletal changes that may aid cell migration. This keratin pair offers the most sensitive marker for altered epidermis following wounding.
确定植入异体薄片的深部手术伤口所发生的结构变化、其时间进程及相互关系。
对19例纹身剃除术后的深部手术伤口（直至真皮深层/皮下脂肪）用性别不匹配的异体角质形成细胞薄片进行治疗，然后每周进行活检直至伤口完全愈合。对20个标准皮肤移植供区等更表浅的手术伤口，在应用异体角质形成细胞移植后7至10天（均已愈合）进行活检。所有活检标本均用一大组针对角蛋白、包膜蛋白、基底膜成分及细胞外基质成分的单克隆抗体进行检测。
增殖性角蛋白对K6/16在所有伤口中均有表达，在植入角质形成细胞的深部伤口中持续表达长达六周，在中厚皮片移植伤口中持续表达长达六个月。
尽管在正常皮肤中未检测到角蛋白6和16，但相关mRNA已被检测到。这增加了在转录后水平进行调控的可能性，从而允许对损伤做出快速反应，伴随细胞骨架变化，这可能有助于细胞迁移。这对角蛋白对是伤口后表皮改变最敏感的标志物。
