Pellegrini G, Bondanza S, Guerra L, De Luca M
Laboratory of Tissue Engineering, I.D.C.-IRCCS, Istituto Dermopatico dell'Immacolata, Pomezia, Roma, Italy.
Med Biol Eng Comput. 1998 Nov;36(6):778-90. doi: 10.1007/BF02518885.
Cultured human keratinocytes have a wide spectrum of clinical applications. Clinical results reported by several investigators are, however, contradictory. In this review, the authors discuss the biological and surgical issues which play a key role in the clinical outcome of cultured epidermal autografts used for the treatment of massive full-thickness burns. The importance of cultivation of epidermal stem cells and of their transplantation onto a wound bed prepared with donor dermis is emphasised. The paper also reviews recent data showing that: (i) cultured epidermal autografts bearing melanocytes can be used for the treatment of stable vitiligo; (ii) keratinocytes isolated from other lining epithelia, such as oral, urethral and corneal epithelia, can be cultivated and grafted onto patients suffering from disabling epithelial defects; (iii) keratinocyte stem cells can be stably transduced with retroviral vectors and are therefore attractive targets for the gene therapy of genodermatoses.
培养的人角质形成细胞具有广泛的临床应用。然而，几位研究者报告的临床结果相互矛盾。在这篇综述中，作者讨论了在用于治疗大面积全层烧伤的培养表皮自体移植临床结果中起关键作用的生物学和外科问题。强调了表皮干细胞培养及其移植到用供体真皮制备的伤口床上的重要性。本文还综述了最近的数据，这些数据表明：（i）携带黑素细胞的培养表皮自体移植可用于治疗稳定期白癜风；（ii）从其他内衬上皮，如口腔、尿道和角膜上皮分离的角质形成细胞可进行培养并移植到患有致残性上皮缺损的患者身上；（iii）角质形成细胞干细胞可用逆转录病毒载体进行稳定转导，因此是遗传性皮肤病基因治疗的有吸引力的靶点。
