Human Nutrition Laboratory, Institute of Food, Nutrition, and Health, ETH Zurich, Zurich, Switzerland.
J Nutr. 2014 Feb;144(2):193-201. doi: 10.3945/jn.113.185561. Epub 2013 Dec 18.
Oxidative stress and low-grade systemic inflammation may contribute to the pathogenesis of obesity-induced comorbidities, including nonalcoholic fatty liver disease. Increasing intake of dietary antioxidants might be beneficial, but there are few data in obese children. To examine the effect of antioxidant supplementation on biomarkers of oxidative stress, inflammation, and liver function, we randomly assigned overweight or obese children and adolescents (n = 44; mean ± SD age: 12.7 ± 1.5 y) participating in a lifestyle modification program to a 4-mo intervention with daily antioxidants (vitamin E, 400 IU; vitamin C, 500 mg; selenium, 50 μg) or placebo. We measured anthropometrics, antioxidant status, oxidative stress (F(2)-isoprostanes, F(2)-isoprostane metabolites), inflammation, liver enzymes, fasting insulin and glucose, and lipid profile at baseline and endpoint. There was a significant treatment effect of antioxidant supplementation on antioxidant status [α-tocopherol, β = 23.2 (95% CI: 18.0, 28.4); ascorbic acid, β = 70.6 (95% CI: 51.7, 89.4); selenium, β = 0.07 (95% CI: 0.01, 0.12)] and oxidative stress [8-iso-prostaglandin F2α, β = -0.11 (95% CI: -0.19, -0.02)] but not on any of the inflammatory markers measured. There was a significant treatment effect on alanine aminotransferase [β = -0.13 (95% CI: -0.23, -0.03)], a trend toward a significant effect on aspartate aminotransferase [β = -0.04 (95% CI: -0.09, 0.01)], and no significant effect on γ-glutamyltransferase [β = -0.03 (95% CI: -0.11, 0.06)]. In summary, antioxidant supplementation for 4 mo improved antioxidant-oxidant balance and modestly improved liver function tests; however, it did not reduce markers of systemic inflammation despite significant baseline correlations between oxidative stress and inflammation. The study was registered at clinicaltrials.gov as NCT01316081.
氧化应激和低度全身炎症可能导致肥胖相关并发症的发病机制，包括非酒精性脂肪肝疾病。增加膳食抗氧化剂的摄入可能是有益的，但在肥胖儿童中数据很少。为了研究抗氧化剂补充对氧化应激、炎症和肝功能生物标志物的影响，我们随机分配超重或肥胖的儿童和青少年（n = 44；平均 ± 标准差年龄：12.7 ± 1.5 岁）参加生活方式改变计划，进行为期 4 个月的干预，每天服用抗氧化剂（维生素 E，400 IU；维生素 C，500 mg；硒，50 μg）或安慰剂。我们在基线和终点测量了人体测量学、抗氧化状态、氧化应激（F2-异前列腺素、F2-异前列腺素代谢物）、炎症、肝酶、空腹胰岛素和血糖以及血脂谱。抗氧化剂补充的治疗效果有显著差异[α-生育酚，β=23.2（95%置信区间：18.0，28.4）；抗坏血酸，β=70.6（95%置信区间：51.7，89.4）；硒，β=0.07（95%置信区间：0.01，0.12）]和氧化应激[8-异前列腺素 F2α，β=-0.11（95%置信区间：-0.19，-0.02）]，但对测量的任何炎症标志物均无显著影响。丙氨酸氨基转移酶[β=-0.13（95%置信区间：-0.23，-0.03）]有显著的治疗效果，天门冬氨酸氨基转移酶[β=-0.04（95%置信区间：-0.09，0.01）]有显著的治疗效果趋势，γ-谷氨酰转移酶[β=-0.03（95%置信区间：-0.11，0.06）]无显著影响。总之，4 个月的抗氧化剂补充改善了抗氧化-氧化平衡，肝功能试验也有所改善；然而，尽管氧化应激和炎症之间存在显著的基线相关性，但它并没有降低全身炎症标志物。该研究在 clinicaltrials.gov 上注册为 NCT01316081。
