Reiser G, Höpp H P, Hamprecht B
Brain Res. 1987 Jan 27;402(1):164-7. doi: 10.1016/0006-8993(87)91061-4.
Atrial natriuretic hormones (ANHs) applied to polyploid rat glioma cells induced hyperpolarizations of about 30 s duration, followed by depolarizations lasting 1-2 min. Repeated applications of the peptide resulted in desensitization. The reversal potential of -87 mV at an extracellular K+ concentration of 5 mM and the decrease of membrane resistance during the hyperpolarization indicate that K+ channels were activated by ANH. In these cells the fluorescence signal of 2-[(2-bis[carboxymethyl]amino-5-methylphenoxy)-methyl]-6-methoxy-8-bis [carboxymethyl]aminoquinoline (quin2) was not affected by ANH suggesting that ANH did not change the cytosolic Ca2+-activity.
将心房利钠激素（ANHs）应用于多倍体大鼠胶质瘤细胞会诱导持续约30秒的超极化，随后是持续1 - 2分钟的去极化。重复应用该肽会导致脱敏。在细胞外钾离子浓度为5 mM时，-87 mV的反转电位以及超极化期间膜电阻的降低表明钾离子通道被ANH激活。在这些细胞中，2-[(2-双[羧甲基]氨基-5-甲基苯氧基)-甲基]-6-甲氧基-8-双[羧甲基]氨基喹啉（quin2）的荧光信号不受ANH影响，这表明ANH不会改变胞质钙离子活性。
