马来西亚国民大学医学中心丙型肝炎病毒基因型及相关危险因素分析。
Hepatitis C genotype and associated risks factors of patients at University Kebangsaan Malaysia Medical Centre.
机构信息
N.A. Mohamed, Faculty of Medicine & Health Sciences, University Sains Islam Malaysia, Kuala Lumpur, Malaysia.
Z. Zainol Rashid, K.K. Wong, Department of Medical Microbiology & Immunology, Faculty of Medicine, Department of Medical Microbiology & Immunology, University Kebangsaan Malaysia Medical Centre (UKMMC), Kuala Lumpur, Malaysia.
出版信息
Pak J Med Sci. 2013 Sep;29(5):1142-6. doi: 10.12669/pjms.295.3610.
OBJECTIVES
Hepatitis C virus (HCV) genotyping is important for treatment and epidemiological purposes. The objective was to determine HCV genotype and their associations with certain risk factors at University Kebangsaan Malaysia Medical Centre (UKMMC).
METHODS
A total of 89 samples were collected from December 2009 to January 2011. Demographic data of patients were collected from medical record. Reverse Transcriptase Polymerase chain reaction (RT PCR) was performed and sixty-four samples yielded positive for HCV. Sequencing was performed and analyzed based on sequence information in GenBank. Statistical analysis were done using SPSS version 15. Results : HCV genotype 3 (73%) was the most frequent genotype, followed by genotype 1(27%). The distribution of HCV genotype/ subtype was as follows: 3a (64.8%), 1a (13.5%), 1 (10.8%), 3 (8.1%) and 1b (2.7%).
CONCLUSIONS
HCV subtypes 3a, 1a, and 1b were identified in patients at UKMMC, Malaysia with subtype 3a being the most prevalent. No significant association was found between HCV genotypes and patients' demographic data.
目的
丙型肝炎病毒（HCV）基因分型对于治疗和流行病学目的非常重要。本研究旨在确定马来西亚国民大学医学中心（UKMMC）HCV 基因型及其与某些危险因素的关联。
方法
本研究共收集了 2009 年 12 月至 2011 年 1 月期间的 89 份样本。从病历中收集了患者的人口统计学数据。采用逆转录聚合酶链反应（RT-PCR）检测，其中 64 份样本 HCV 呈阳性。对 HCV 阳性样本进行测序，并根据 GenBank 中的序列信息进行分析。采用 SPSS 版本 15 进行统计分析。结果：最常见的 HCV 基因型为 3 型（73%），其次为 1 型（27%）。HCV 基因型/亚型的分布如下：3a（64.8%）、1a（13.5%）、1（10.8%）、3（8.1%）和 1b（2.7%）。
结论
在马来西亚 UKMMC 的患者中发现了 HCV 亚型 3a、1a 和 1b，其中 3a 亚型最为常见。HCV 基因型与患者的人口统计学数据之间未发现显著关联。
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