Rozanov Mikhail, Plikat Uwe, Chappey Colombe, Kochergin Andrey, Tatusova Tatiana
National Center for Biotechnology Information, National Library of Medicine, National Institute of Health, Building 38A, Room S602, 8600 Rockville Pike, Bethesda, MD 20892, USA.
Nucleic Acids Res. 2004 Jul 1;32(Web Server issue):W654-9. doi: 10.1093/nar/gkh419.
The Genotyping tool at the National Center for Biotechnology Information is a web-based program that identifies the genotype (or subtype) of recombinant or non-recombinant viral nucleotide sequences. It works by using BLAST to compare a query sequence to a set of reference sequences for known genotypes. Predefined reference genotypes exist for three major viral pathogens: human immunodeficiency virus 1 (HIV-1), hepatitis C virus (HCV) and hepatitis B virus (HBV). User-defined reference sequences can be used at the same time. The query sequence is broken into segments for comparison to the reference so that the mosaic organization of recombinant sequences could be revealed. The results are displayed graphically using color-coded genotypes. Therefore, the genotype(s) of any portion of the query can quickly be determined. The Genotyping tool can be found at: http://www.ncbi.nih.gov/projects/genotyping/formpage.cgi.
美国国立生物技术信息中心的基因分型工具是一个基于网络的程序，可识别重组或非重组病毒核苷酸序列的基因型（或亚型）。它通过使用BLAST将查询序列与一组已知基因型的参考序列进行比较来工作。针对三种主要病毒病原体存在预定义的参考基因型：人类免疫缺陷病毒1型（HIV-1）、丙型肝炎病毒（HCV）和乙型肝炎病毒（HBV）。也可以同时使用用户定义的参考序列。查询序列会被分成片段与参考序列进行比较，以便揭示重组序列的镶嵌结构。结果使用颜色编码的基因型以图形方式显示。因此，可以快速确定查询序列任何部分的基因型。基因分型工具可在以下网址找到：http://www.ncbi.nih.gov/projects/genotyping/formpage.cgi 。
