Khaire Amrita, Rathod Richa, Kemse Nisha, Kale Anvita, Joshi Sadhana
Interactive Research School for Health Affairs (IRSHA), Bharati Vidyapeeth Deemed University, Pune - 411043, India.
Reprod Fertil Dev. 2015 Jan;27(2):341-50. doi: 10.1071/RD13306.
Maternal vitamin B12 deficiency leads to an adverse pregnancy outcome and increases the risk for developing diabetes and metabolic syndrome in mothers in later life. Our earlier studies have demonstrated that vitamin B12 and n-3 polyunsaturated fatty acids (PUFA) are interlinked in the one carbon cycle. The present study for the first time examines the effect of maternal n-3 PUFA supplementation to vitamin B12 deficient or supplemented diets on pregnancy outcome, fatty-acid status and metabolic variables in Wistar rats. Pregnant dams were assigned to one of the following groups: control, vitamin B12 deficient, vitamin B12 supplemented, vitamin B12 deficient + n-3 PUFA or vitamin B12 supplemented + n-3 PUFA. The amount of vitamin B12 in the supplemented group was 0.50 μg kg(-1) diet and n-3 PUFA was alpha linolenic acid (ALA) 1.68, eicosapentaenoic acid 5.64, docosahexaenoic acid (DHA) 3.15 (g per 100g fatty acids per kg diet). Our findings indicate that maternal vitamin B12 supplementation did not affect the weight gain of dams during pregnancy but reduced litter size and weight and was ameliorated by n-3 PUFA supplementation. Vitamin B12 deficiency or supplementation resulted in a low percentage distribution of plasma arachidonic acid and DHA. n-3 PUFA supplementation to these diets improved the fatty-acid status. Vitamin B12 deficiency resulted in higher homocysteine and insulin levels, which were normalised by supplementation with either vitamin B12 or n-3 PUFA. Our study suggests that maternal vitamin B12 status is critical in determining pregnancy outcome and metabolic variables in dams and that supplementation with n-3 PUFA is beneficial.
母体维生素B12缺乏会导致不良妊娠结局，并增加母亲晚年患糖尿病和代谢综合征的风险。我们早期的研究表明，维生素B12和n-3多不饱和脂肪酸（PUFA）在一碳循环中相互关联。本研究首次考察了在Wistar大鼠中，母体补充n-3 PUFA对维生素B12缺乏或补充饮食的妊娠结局、脂肪酸状态和代谢变量的影响。将怀孕的母鼠分为以下几组之一：对照组、维生素B12缺乏组、维生素B12补充组、维生素B12缺乏 + n-3 PUFA组或维生素B12补充 + n-3 PUFA组。补充组中维生素B12的含量为0.50μg/kg饮食，n-3 PUFA为α-亚麻酸（ALA）1.68、二十碳五烯酸5.64、二十二碳六烯酸（DHA）3.15（每千克饮食中每100g脂肪酸中的克数）。我们的研究结果表明，母体补充维生素B12不会影响母鼠孕期体重增加，但会降低窝仔数和体重，而补充n-3 PUFA可改善这种情况。维生素B12缺乏或补充会导致血浆花生四烯酸和DHA的分布百分比降低。在这些饮食中补充n-3 PUFA可改善脂肪酸状态。维生素B12缺乏会导致同型半胱氨酸和胰岛素水平升高，补充维生素B12或n-3 PUFA可使其恢复正常。我们的研究表明，母体维生素B12状态对决定母鼠的妊娠结局和代谢变量至关重要，补充n-3 PUFA有益。
