从澳大利亚昆士兰州的一只灰噪鸦（Cracticus torquatus）中分离出的伯劳鸟多瘤病毒。

Butcherbird polyomavirus isolated from a grey butcherbird (Cracticus torquatus) in Queensland, Australia.

机构信息

School of Veterinary and Life Sciences, Murdoch University, South Street, Murdoch 6150, WA, Australia.

Australia Zoo Wildlife Hospital, Beerwah 4519, QLD, Australia.

出版信息

Vet Microbiol. 2014 Jan 31;168(2-4):302-11. doi: 10.1016/j.vetmic.2013.11.026. Epub 2013 Nov 28.

DOI:10.1016/j.vetmic.2013.11.026

PMID:24355535

Abstract

A novel avian polyomavirus was detected in peri-ocular skin lesions collected from a grey butcherbird (Cracticus torquatus), using a combination of multiply primed rolling circle amplification, nested PCR and long range PCR. The sequence of Butcherbird polyomavirus was determined by combining next generation sequencing and primer walking techniques. The circular double-stranded DNA genome of Butcherbird polyomavirus consisted of 5084 bp, and encoded six open reading frames (ORF-X, VP2, VP3, VP1, small T-antigen and large T-antigen). Phylogenetic analysis placed it amongst other members of the genus Avipolyomavirus, most closely related to Crow polyomavirus. Next generation sequencing enabled the detection of DNA fragments similar to, but distinct from, Canarypox virus within the same lesion from which Butcherbird polyomavirus was amplified, thus confirming an avipolyomavirus-avipoxvirus co-infection in the peri-ocular skin lesions of this grey butcherbird.

摘要

一种新型禽多瘤病毒在从灰噪鸦（Cracticus torquatus）眼部周围皮肤病变中采集的样本中被检测到，该方法结合了多次引物滚环扩增、巢式 PCR 和长距离 PCR。通过结合下一代测序和引物行走技术确定了灰噪鸦多瘤病毒的序列。灰噪鸦多瘤病毒的环状双链 DNA 基因组由 5084 个碱基对组成，编码六个开放阅读框（ORF-X、VP2、VP3、VP1、小 T 抗原和大 T 抗原）。系统发育分析将其置于其他属的禽多瘤病毒中，与鸦科多瘤病毒最为密切相关。下一代测序能够在从扩增出灰噪鸦多瘤病毒的同一病变中检测到与金丝雀痘病毒相似但又不同的 DNA 片段，从而证实了这种灰噪鸦眼部周围皮肤病变中存在禽多瘤病毒和禽痘病毒的混合感染。