de Sales Lima Francisco Esmaile, Cibulski Samuel Paulo, Witt André Alberto, Franco Ana Cláudia, Roehe Paulo Michel
Virology Laboratory, Department of Microbiology, Immunology and Parasitology, Institute of Basic Health Sciences, Federal University of Rio Grande do Sul (UFRGS), Rua Sarmento Leite 500, Porto Alegre, Rio Grande do Sul, CEP 90050-170, Brazil,
Arch Virol. 2015 Jul;160(7):1831-6. doi: 10.1007/s00705-015-2447-6. Epub 2015 May 12.
Two novel genomes comprising ≈4.9 kb were identified by next-generation sequencing from pooled organs of Tadarida brasiliensis bats. The overall nucleotide sequence identities between the viral genomes characterized here were less than 80% in comparison to other polyomaviruses (PyVs), members of the family Polyomaviridae. The new genomes display the archetypal organization of PyVs, which includes open reading frames for the regulatory proteins small T antigen (STAg) and large T antigen (LTAg), as well as capsid proteins VP1, VP2 and VP3. In addition, an alternate ORF was identified in the early genome region that is conserved in a large monophyletic group of polyomaviruses. Phylogenetic analysis showed similar clustering with group of PyVs detected in Otomops and Chaerephon bats and some species of monkeys. In this study, the genomes of two novel PyVs were detected in bats of a single species, demonstrating that these mammals can harbor genetically diverse polyomaviruses.
通过下一代测序技术，从巴西犬吻蝠的合并器官中鉴定出了两个约4.9 kb的新基因组。与多瘤病毒科的其他多瘤病毒（PyV）相比，此处鉴定的病毒基因组之间的总体核苷酸序列同一性低于80%。新基因组展示了多瘤病毒的典型结构，包括调节蛋白小T抗原（STAg）和大T抗原（LTAg）以及衣壳蛋白VP1、VP2和VP3的开放阅读框。此外，在早期基因组区域鉴定出一个替代开放阅读框，该区域在一大类单系多瘤病毒中保守。系统发育分析表明，与在食果蝠和非洲犬吻蝠以及一些猴类物种中检测到的多瘤病毒组聚类相似。在本研究中，在单一物种的蝙蝠中检测到两种新型多瘤病毒的基因组，表明这些哺乳动物可以携带基因多样的多瘤病毒。
