体外芽再生的分子途径。
The molecular path to in vitro shoot regeneration.
机构信息
Department of Applied Biosciences, Faculty of Bioscience Engineering, Ghent University, V. Vaerwyckweg 1, BE-9000 Ghent, Belgium; Department of Plant Production, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, BE-9000 Ghent, Belgium.
Department of Applied Biosciences, Faculty of Bioscience Engineering, Ghent University, V. Vaerwyckweg 1, BE-9000 Ghent, Belgium.
出版信息
Biotechnol Adv. 2014 Jan-Feb;32(1):107-21. doi: 10.1016/j.biotechadv.2013.12.002. Epub 2013 Dec 16.
Plant regeneration through de novo shoot organogenesis in tissue culture is a critical step in most plant transformation and micropropagation procedures. Establishing an efficient regeneration protocol is an empirical process and requires optimization of multiple factors that influence the regeneration capacity. Here, we review the molecular process of shoot induction in a two-step regeneration protocol and focus on the role of auxins and cytokinins. First, during incubation on an auxin-rich callus induction medium (CIM), organogenic callus is produced that exhibits characteristics of a root meristem. Subsequent incubation on a cytokinin-rich shoot induction medium (SIM) induces root to shoot conversion. Through a detailed analysis of the different aspects of shoot regeneration, we try to reveal hinge points and novel candidate genes that may be targeted to increase shoot regeneration capacity in order to improve transformation protocols.
通过组织培养中的从头芽器官发生进行植物再生是大多数植物转化和微繁殖程序中的关键步骤。建立有效的再生方案是一个经验性过程，需要优化影响再生能力的多个因素。在这里，我们回顾了两步再生方案中芽诱导的分子过程，并重点介绍了生长素和细胞分裂素的作用。首先，在富含生长素的愈伤组织诱导培养基（CIM）上培养时，会产生具有根分生组织特征的器官发生愈伤组织。随后在富含细胞分裂素的芽诱导培养基（SIM）上培养会诱导根到芽的转化。通过对芽再生的不同方面进行详细分析，我们试图揭示可能成为提高芽再生能力的靶点的关键点和新的候选基因，以改进转化方案。
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