Temman Haruka, Sakamoto Takuya, Ueda Minoru, Sugimoto Kaoru, Migihashi Masako, Yamamoto Kazunari, Tsujimoto-Inui Yayoi, Sato Hikaru, Shibuta Mio K, Nishino Norikazu, Nakamura Tomoe, Shimada Hiroaki, Taniguchi Yukimi Y, Takeda Seiji, Aida Mitsuhiro, Suzuki Takamasa, Seki Motoaki, Matsunaga Sachihiro
Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278-8510, Japan.
Plant Genomic Network Research Team, RIKEN Center for Sustainable Resource Science, 1-7-22 Suehiro, Tsurumi, Yokohama, Kanagawa 230-0045, Japan.
PNAS Nexus. 2023 Jan 6;2(2):pgad002. doi: 10.1093/pnasnexus/pgad002. eCollection 2023 Feb.
During de novo plant organ regeneration, auxin induction mediates the formation of a pluripotent cell mass called callus, which regenerates shoots upon cytokinin induction. However, molecular mechanisms underlying transdifferentiation remain unknown. Here, we showed that the loss of , a histone deacetylase (HDAC) family gene, suppresses shoot regeneration. Treatment with an HDAC inhibitor revealed that the activity of this gene is essential for shoot regeneration. Further, we identified target genes whose expression was regulated through HDA19-mediated histone deacetylation during shoot induction and found that and play important roles in shoot apical meristem formation. Histones at the loci of these genes were hyperacetylated and markedly upregulated in . Transient or overexpression impaired shoot regeneration, as observed in . Therefore, HDA19 mediates direct histone deacetylation of and loci to prevent their overexpression at the early stages of shoot regeneration.
在植物从头器官再生过程中，生长素诱导介导了一种称为愈伤组织的多能细胞团的形成，该细胞团在细胞分裂素诱导下再生出芽。然而，转分化的分子机制仍然未知。在这里，我们表明组蛋白脱乙酰酶（HDAC）家族基因的缺失会抑制芽再生。用HDAC抑制剂处理表明该基因的活性对于芽再生至关重要。此外，我们鉴定了在芽诱导过程中其表达通过HDA19介导的组蛋白去乙酰化而受到调控的靶基因，并发现和在茎尖分生组织形成中起重要作用。这些基因位点的组蛋白在中发生了高度乙酰化并显著上调。正如在中观察到的那样，瞬时或过表达会损害芽再生。因此，HDA19介导和基因位点的直接组蛋白去乙酰化，以防止它们在芽再生早期过度表达。
