Mantur M, Wołosowicz N, Prokopowicz J, Kemona H
Folia Haematol Int Mag Klin Morphol Blutforsch. 1986;113(5):685-9.
We have elaborated a system for testing the phagocytic activity of human blood platelets. Blood was sampled to heparin and ACD and centrifuged. This procedure yields platelet rich plasma (PRP). As a substrate for phagocytosis we used Staphylococcus aureus 209P. The ratio of platelets to bacteria is 1:1. Incubation of bacteria and platelets continued up to 10 minutes. Lysostaphine was introduced to the phagocytic system to destroy all bacteria outside the blood platelets. The maximal values for the percentage of phagocytizing blood platelets were 0.7% and the phagocytosis index of 1.0 was reached at 6 minutes with incubation of bacteria and platelets. The authors pay attention to the role of blood platelets in antibacterial mechanisms.
我们精心设计了一个用于检测人类血小板吞噬活性的系统。采集血液并加入肝素和ACD，然后进行离心。此操作可得到富含血小板的血浆（PRP）。作为吞噬作用的底物，我们使用了金黄色葡萄球菌209P。血小板与细菌的比例为1:1。细菌和血小板的孵育持续10分钟。向吞噬系统中加入溶葡萄球菌素以破坏血小板外的所有细菌。吞噬血小板的百分比最大值为0.7%，在细菌与血小板孵育6分钟时达到吞噬指数1.0。作者关注血小板在抗菌机制中的作用。
