Xue Mei, Xuan Yong-Li, Wang Ye, Hu He-Sheng, Li Xiao-Lu, Suo Fei, Li Xin-Ran, Cheng Wen-Juan, Yan Su-Hua
Department of Cardiology, Shandong Provincial Qianfoshan Hospital, Shandong University, Jinan, P.R. China.
Cardiology. 2014;127(3):155-63. doi: 10.1159/000355535. Epub 2013 Dec 12.
Diabetic cardiac autonomic neuropathy can lead to an increased incidence of ventricular arrhythmias (VAs). However, few data are available regarding the pathogenesis and therapy of the VAs accompanying diabetic cardiac autonomic neuropathy. We aimed to explore whether or not exogenous nerve growth factor (NGF) can reduce the sympathetic heterogeneity and the incidence of VAs in diabetes mellitus (DM).
Male Wistar rats were randomly divided into 3 groups: controls, rats with DM with saline infused into the left stellate ganglion (LSG), i.e. the DS group and rats with DM with NGF infused into the LSG, i.e. the DN group. After 28 weeks, all rats were subjected to electrophysiological experiments. Sympathetic innervations and NGF were studied by immunostaining, RT-PCR or Western blot analysis.
The incidence of inducible VAs was significantly higher in the DS group than in the control group, but was markedly decreased in the DN group. In the DS group, the tyrosine hydroxylase (TH) and NGF expression were significantly lower than in the other groups, and significant proximal-distal heterogeneities existed regarding the TH and NGF expression in the left ventricle, but were markedly repaired in the DN group.
NGF intervention in the LSG can reduce the heterogeneity of cardiac sympathetic innervations and the incidence of VAs in diabetic rats.
糖尿病性心脏自主神经病变可导致室性心律失常（VA）的发生率增加。然而，关于糖尿病性心脏自主神经病变伴发VA的发病机制和治疗的数据很少。我们旨在探讨外源性神经生长因子（NGF）是否能降低糖尿病（DM）患者心脏交感神经的异质性和VA的发生率。
雄性Wistar大鼠随机分为3组：对照组、经左星状神经节（LSG）注入生理盐水的糖尿病大鼠（即DS组）和经LSG注入NGF的糖尿病大鼠（即DN组）。28周后，对所有大鼠进行电生理实验。通过免疫染色、RT-PCR或蛋白质印迹分析研究交感神经支配和NGF。
DS组诱发性VA的发生率显著高于对照组，但DN组明显降低。在DS组中，酪氨酸羟化酶（TH）和NGF表达明显低于其他组，左心室TH和NGF表达存在明显的近端-远端异质性，但在DN组中明显修复。
对LSG进行NGF干预可降低糖尿病大鼠心脏交感神经支配的异质性和VA的发生率。
