Science. 2013 Dec 20;342(6165):1241089. doi: 10.1126/science.1241089.
Amborella trichopoda is strongly supported as the single living species of the sister lineage to all other extant flowering plants, providing a unique reference for inferring the genome content and structure of the most recent common ancestor (MRCA) of living angiosperms. Sequencing the Amborella genome, we identified an ancient genome duplication predating angiosperm diversification, without evidence of subsequent, lineage-specific genome duplications. Comparisons between Amborella and other angiosperms facilitated reconstruction of the ancestral angiosperm gene content and gene order in the MRCA of core eudicots. We identify new gene families, gene duplications, and floral protein-protein interactions that first appeared in the ancestral angiosperm. Transposable elements in Amborella are ancient and highly divergent, with no recent transposon radiations. Population genomic analysis across Amborella's native range in New Caledonia reveals a recent genetic bottleneck and geographic structure with conservation implications.
单室木兰强烈支持作为所有现存开花植物姐妹谱系的唯一活种，为推断现存被子植物最近共同祖先（MRCA）的基因组内容和结构提供了独特的参考。通过对单室木兰基因组进行测序，我们确定了一个在被子植物多样化之前发生的古老基因组加倍事件，没有证据表明随后发生了谱系特异性的基因组加倍事件。单室木兰与其他被子植物之间的比较促进了核心真双子叶植物 MRCA 中祖先被子植物基因含量和基因顺序的重建。我们确定了新的基因家族、基因加倍和首次出现在祖先被子植物中的花部蛋白-蛋白相互作用。单室木兰中的转座因子是古老的且高度分化的，没有最近的转座子辐射。在新喀里多尼亚的单室木兰的原生范围内进行的群体基因组分析揭示了最近的遗传瓶颈和地理结构，具有保护意义。
