Wang Yan, Qi Yanni, Wang Limin, Xu Chenmeng, Li Wenjuan, Dang Zhao, Zhao Wei, Wang Ping, Xie Yaping, Niu Yamin, Lu Nan, Hu Zuyu, Liu Zigang, Zhang Jianping
College of Agriculture, Gansu Agricultural University, Lanzhou 730070, China.
Institute of Crop, Gansu Academy of Agricultural Sciences, Lanzhou 730070, China.
Int J Mol Sci. 2025 Mar 27;26(7):3074. doi: 10.3390/ijms26073074.
Dehydration-responsive element-binding () transcription factors are ubiquitous in plants and regulate plant growth, development, signal transduction, and responses to stress, particularly drought stress. However, genes in flax have not previously been studied. This study conducted a comprehensive and systematic analysis of the gene family in flax ( L.). A total of 59 genes were identified in Longya-10 (a breeding variety), with an uneven distribution across all 15 chromosomes. Further analysis revealed significant variations among LuDREB members, with predictions indicating that these proteins are hydrophilic and localized in the nucleus and cytoplasm. A phylogenetic analysis classified the genes into six subgroups, a classification further supported by gene structure and motif composition. Members within the same subgroup exhibited structural conservation, suggesting functional redundancy. The duplication analysis identified 30 pairs of segmentally duplicated genes and one pair of tandemly duplicated genes, indicating that segmental duplication was the primary driver of gene expansion. A comparative collinearity analysis revealed that most genes had orthologs in other plant species, suggesting that this gene family has remained relatively conserved throughout evolution. -acting element analysis identified numerous hormone- and stress-responsive elements in promoters, and the quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR) results confirmed the role of all genes in drought stress response. In addition, transcriptome analysis revealed that and exhibited high expression levels in the capsules, whereas and showed significantly higher expression levels in the stems, suggesting that these genes may have specialized functions in capsule or stem development. Collectively, this study provides a comprehensive overview of genes, offering valuable insights into their roles in flax growth, development, and stress responses.
脱水响应元件结合（DREB）转录因子在植物中普遍存在，调控植物生长、发育、信号转导以及对胁迫的响应，尤其是干旱胁迫。然而，亚麻中的DREB基因此前尚未被研究。本研究对亚麻（Linum usitatissimum L.）中的DREB基因家族进行了全面系统的分析。在龙亚-10（一个育成品种）中总共鉴定出59个DREB基因，它们在全部15条染色体上分布不均。进一步分析揭示了陆地棉DREB成员间存在显著差异，预测表明这些蛋白质具有亲水性，定位于细胞核和细胞质中。系统发育分析将DREB基因分为六个亚组，基因结构和基序组成进一步支持了这一分类。同一亚组内的成员表现出结构保守性，表明功能冗余。重复分析鉴定出30对片段重复的DREB基因和一对串联重复基因，表明片段重复是DREB基因扩张的主要驱动力。比较共线性分析表明，大多数DREB基因在其他植物物种中有直系同源基因，这表明该基因家族在整个进化过程中相对保守。顺式作用元件分析在DREB启动子中鉴定出大量激素和胁迫响应元件，定量实时逆转录聚合酶链反应（qRT-PCR）结果证实了所有DREB基因在干旱胁迫响应中的作用。此外，转录组分析表明，LuDREB1和LuDREB2在蒴果中表达水平较高，而LuDREB3和LuDREB4在茎中表达水平显著更高，这表明这些DREB基因可能在蒴果或茎的发育中具有特定功能。总体而言，本研究提供了对DREB基因的全面概述，为其在亚麻生长、发育和胁迫响应中的作用提供了有价值的见解。
