Morioka S, Lazarus G S, Baird J L, Jensen P J
J Invest Dermatol. 1987 Apr;88(4):418-23. doi: 10.1111/1523-1747.ep12469754.
When confluent keratinocyte cultures were wounded by cutting with a blade, the cells rapidly retracted from the wounded site, leaving an area denuded of cells. Within 3-4 h of wounding, keratinocytes began to migrate from the edges and gradually reepithelialized the entire denuded area. Mitomycin C did not prevent the reepithelialization but did dramatically inhibit [3H]thymidine incorporation into the leading edge of cells. These results indicate that cell proliferation was not required for reepithelialization. Using a rabbit antibody against urokinase-type plasminogen activator (u-PA) and an avidin-biotin-peroxidase detection method, we localized u-PA in the keratinocytes at the leading edge of the migrating cultures. Cytochalasin B dramatically inhibited the extent of migration and also altered cell morphology; nonetheless, urokinase was detected in the limited number of cells that moved into the wounded area, even in the presence of cytochalasin B. A small but consistent enhancement (36% +/- 9) of plasminogen activator activity was observed in the supernatant of wounded cultures. These data suggest that plasminogen activator may be involved in the migration of keratinocytes that occurs during wound healing.
当用刀片切割使角质形成细胞汇合培养物受伤时，细胞迅速从受伤部位回缩，留下一个无细胞的区域。在受伤后3 - 4小时内，角质形成细胞开始从边缘迁移，并逐渐重新上皮化整个无细胞区域。丝裂霉素C并没有阻止重新上皮化，但确实显著抑制了[3H]胸苷掺入细胞前沿。这些结果表明重新上皮化不需要细胞增殖。使用抗尿激酶型纤溶酶原激活剂（u-PA）的兔抗体和抗生物素蛋白-生物素-过氧化物酶检测方法，我们将u-PA定位在迁移培养物前沿的角质形成细胞中。细胞松弛素B显著抑制迁移程度并改变细胞形态；尽管如此，即使在存在细胞松弛素B的情况下，在迁移到受伤区域的有限数量细胞中仍检测到尿激酶。在受伤培养物的上清液中观察到纤溶酶原激活剂活性有小幅但一致的增强（36%±9）。这些数据表明纤溶酶原激活剂可能参与伤口愈合过程中发生的角质形成细胞迁移。
