Sinha S, Gupta S K, Sengupta U
Int J Lepr Other Mycobact Dis. 1987 Mar;55(1):42-53.
A variety of mycobacterial antigens have been associated with the delayed-type hypersensitivity (DTH) reactions in human beings and animals. An attempt has been made in the present study to identify the major antigen fractions of Mycobacterium leprae and M. tuberculosis responsible for the elicitation of DTH and to evaluate their specificity by a comparison with the antigens of two closely related mycobacteria, BCG and M. vaccae. Guinea pigs and mice were sensitized with native or heat-killed M. leprae, M. tuberculosis, M. vaccae, and BCG. Four fractions each of the latter three mycobacteria, obtained by preparatory polyacrylamide slab gel electrophoresis, were used for elicitation of DTH. Three fractions of M. leprae (MY1, MY2, and MLSE) were obtained by affinity chromatography using monoclonal antibodies. Heat-killed M. leprae and M. vaccae proved to be better sensitizers for DTH compared to their native counterparts, whereas there were no significant differences between the efficacies of autoclaved and unautoclaved M. tuberculosis or BCG. The best DTH responses were recorded with a 16-24 kD fraction of M. tuberculosis, a 20-27 kD fraction of BCG, and MLSE of M. leprae. The purified molecules of M. leprae, MY1 (12 kD) and MY2 (35 kD), did not elicit a DTH reaction. Although none of the fractions of M. tuberculosis or BCG exhibited species specificity, fraction II (15-24 kD) of M. vaccae and MLSE might be comprised of species-specific determinants.
多种分枝杆菌抗原已与人及动物的迟发型超敏反应（DTH）相关。本研究试图鉴定引起DTH的麻风分枝杆菌和结核分枝杆菌的主要抗原组分，并通过与两种密切相关的分枝杆菌卡介苗（BCG）和母牛分枝杆菌（M. vaccae）的抗原进行比较来评估它们的特异性。用天然或热灭活的麻风分枝杆菌、结核分枝杆菌、母牛分枝杆菌和卡介苗对豚鼠和小鼠进行致敏。通过制备性聚丙烯酰胺平板凝胶电泳获得的后三种分枝杆菌的各四个组分用于引发DTH。使用单克隆抗体通过亲和层析获得了麻风分枝杆菌的三个组分（MY1、MY2和MLSE）。与天然的相比，热灭活的麻风分枝杆菌和母牛分枝杆菌被证明是更好的DTH致敏剂，而高压灭菌和未高压灭菌的结核分枝杆菌或卡介苗的效力之间没有显著差异。结核分枝杆菌的16 - 24 kD组分、卡介苗的20 - 27 kD组分和麻风分枝杆菌的MLSE记录到了最佳的DTH反应。纯化的麻风分枝杆菌分子MY1（12 kD）和MY2（35 kD）未引发DTH反应。虽然结核分枝杆菌或卡介苗的任何组分都没有表现出种属特异性，但母牛分枝杆菌的组分II（15 - 24 kD）和MLSE可能包含种属特异性决定簇。
