Bursch W, Schulte-Hermann R
Klin Wochenschr. 1986;64 Suppl 7:47-50.
It was investigated whether the prostacyclin derivative Iloprost (Schering, Berlin) protects rat hepatocytes against lethal damage induced by carbon tetrachloride (CCl4) and bromobenzene (BB). Iloprost was tested in whole animal experiments (intoxication with 2 ml CCl4/kg) and with primary hepatocyte cultures (intoxication with 1.6 mM BB). Cell damage was estimated by light microscopic examination of hepatocellular morphology and by the release of hepatocellular enzymes (glutamic-pyruvic transaminase, GPT; glutamic-oxalacetic transaminase, GOT; lactic dehydrogenase, LDH) into the blood or culture medium. In both experimental set-ups, Iloprost (0.1 micrograms/kg/min in whole animal experiments and 10(-9)-10(-12) M in primary hepatocyte cultures) largely preserved normal hepatocellular morphology after intoxication. Furthermore, the toxin-induced release of hepatocellular enzymes into the blood (GOT, GPT) or into the culture medium (LDH) was reduced by 50%-70% in the presence of Iloprost. It is concluded that the prostacyclin derivative Iloprost possesses cytoprotective activity on rat hepatocytes against lethal injury by CCl4 or BB.
研究了前列环素衍生物依洛前列素（先灵公司，柏林）是否能保护大鼠肝细胞免受四氯化碳（CCl4）和溴苯（BB）诱导的致命损伤。依洛前列素在整体动物实验（2 ml CCl4/kg中毒）和原代肝细胞培养实验（1.6 mM BB中毒）中进行了测试。通过光学显微镜检查肝细胞形态以及肝细胞酶（谷丙转氨酶，GPT；谷草转氨酶，GOT；乳酸脱氢酶，LDH）释放到血液或培养基中的情况来评估细胞损伤。在这两种实验设置中，依洛前列素（整体动物实验中为0.1微克/千克/分钟，原代肝细胞培养中为10^(-9)-10^(-12) M）在中毒后在很大程度上保持了正常的肝细胞形态。此外，在依洛前列素存在的情况下，毒素诱导的肝细胞酶释放到血液（GOT、GPT）或培养基（LDH）中的量减少了50%-70%。得出的结论是，前列环素衍生物依洛前列素对大鼠肝细胞具有抗CCl4或BB所致致命损伤的细胞保护活性。
