Ophir D, Marshak G, Czernobilsky B
Laryngoscope. 1987 Apr;97(4):490-4. doi: 10.1288/00005537-198704000-00015.
A laryngeal squamous cell carcinoma in situ with an underlying spindle cell nodule (pseudosarcoma) was immunohistochemically labeled with antibodies to tissue-specific intermediate filament proteins, including desmin, vimentin, and cytokeratin. Two distinct populations of cells were found within the lesion: cytokeratin-positive cells, corresponding to the carcinomatous component of the tumor, and vimentin-positive spindle cells in the subepithelial nodule. In view of the strict specificity of antivimentin and anticytokeratin for cells of mesenchymal and epithelial origin, respectively, it is proposed that the two components of the pseudosarcoma in our case are not morphologic variants of the same tumor, and that the subepithelial nodule represents a mesenchymal lesion. These results can, however, not be extrapolated to other cases since in some the spindle cell component may represent metaplastic epithelial cells. In view of the difficulties encountered in reaching a correct diagnosis in these lesions, it is recommended to use intermediate filament typing to elucidate the nature of the spindle cells in this controversial tumor.
一例伴有潜在梭形细胞结节（假肉瘤）的原位喉鳞状细胞癌，用针对组织特异性中间丝蛋白（包括结蛋白、波形蛋白和细胞角蛋白）的抗体进行免疫组织化学标记。在病变中发现了两种不同类型的细胞：细胞角蛋白阳性细胞，对应于肿瘤的癌性成分；以及上皮下结节中波形蛋白阳性的梭形细胞。鉴于抗波形蛋白和抗细胞角蛋白分别对间充质来源和上皮来源的细胞具有严格的特异性，我们认为本病例中假肉瘤的两个成分并非同一肿瘤的形态学变异，上皮下结节代表间充质病变。然而，这些结果不能外推至其他病例，因为在某些病例中，梭形细胞成分可能代表化生的上皮细胞。鉴于在对这些病变做出正确诊断时遇到困难，建议使用中间丝分型来阐明这种有争议肿瘤中梭形细胞的性质。
