College of Veterinary Medicine, Northwest A&F University, Yangling, P. R. China; Shaanxi Center of Stem Cell Engineering & Technology, Yangling, P. R. China; Department of Life Science and Technology, Ningxia Polytechnic, Yinchuan, P. R. China.
College of Veterinary Medicine, Northwest A&F University, Yangling, P. R. China; Shaanxi Center of Stem Cell Engineering & Technology, Yangling, P. R. China.
Theriogenology. 2014 Mar 1;81(4):535-44. doi: 10.1016/j.theriogenology.2013.11.005. Epub 2013 Nov 11.
The cell division cycle protein 20 (CDC20) is an essential regulator of cell division, encoded by the CDC20 gene. However, the role of CDC20 in bovine oocyte maturation is unknown. In this study, CDC20 morpholino antisense oligonucleotides (MOs) were microinjected into the cytoplasm of bovine oocytes to block the translation of CDC20 mRNA. CDC20 downregulation significantly reduced the rate of first polar body emission (PB1). Further analysis indicated that oocytes treated with CDC20 MO arrested before or at meiotic stage I with abnormal spindles. To further confirm the functions of CDC20 during oocyte meiotic division, CDC20 MOs were microinjected into oocytes together with a supplementary PB1. The results showed that newly synthesized CDC20 was not necessary at the meiosis II-to-anaphase II transition. Our data suggest that CDC20 is required for spindle assembly, chromosomal segregation, and PB1 extrusion during bovine oocyte maturation.
细胞分裂周期蛋白 20（CDC20）是细胞分裂的必需调节因子，由 CDC20 基因编码。然而，CDC20 在牛卵母细胞成熟中的作用尚不清楚。在这项研究中，将 CDC20 形态发生素反义寡核苷酸（MOs）显微注射到牛卵母细胞的细胞质中，以阻断 CDC20 mRNA 的翻译。CDC20 下调显著降低了第一次极体排放（PB1）的速率。进一步的分析表明，用 CDC20 MO 处理的卵母细胞在减数分裂 I 期之前或期间停滞，纺锤体异常。为了进一步证实 CDC20 在卵母细胞减数分裂中的功能，将 CDC20 MO 与补充的 PB1 一起显微注射到卵母细胞中。结果表明，在减数分裂 II 到后期 II 的转变过程中，新合成的 CDC20 不是必需的。我们的数据表明，CDC20 对于牛卵母细胞成熟过程中的纺锤体组装、染色体分离和 PB1 挤出是必需的。
