Central Research Institute, Maruha Nichiro Holdings, Inc., 16-2 Wadai, Tsukuba, Ibaraki 300-4295, Japan.
Central Research Institute, Maruha Nichiro Holdings, Inc., 16-2 Wadai, Tsukuba, Ibaraki 300-4295, Japan.
Food Chem. 2014 May 1;150:348-52. doi: 10.1016/j.foodchem.2013.10.130. Epub 2013 Nov 4.
We developed and validated a novel lateral flow assay for the detection of crustacean protein in processed foods. This assay had high sensitivity; the visual detection limit for shrimp protein extract was 25μg/L, equivalent to 1μg/g protein in a food sample, and results could be obtained within 20min without sophisticated procedures or expensive equipment. Concordance between our assay and another validated quantitative enzyme-linked immunosorbent assay was 97% for commercially processed foods. This assay is rapid, simple, reliable, and highly correlated with validated enzyme-linked immunosorbent assays and is thus suitable for monitoring of food products, especially in food-processing facilities.
我们开发并验证了一种用于检测加工食品中甲壳类蛋白的新型侧向流动分析方法。该方法具有很高的灵敏度；虾蛋白提取物的视觉检测限为 25μg/L，相当于食品样品中 1μg/g 蛋白质，并且可以在 20min 内获得结果，无需复杂的程序或昂贵的设备。我们的测定方法与另一种经过验证的定量酶联免疫吸附测定法在商业加工食品中的符合率为 97%。该测定方法快速、简单、可靠，与经过验证的酶联免疫吸附测定法高度相关，因此适用于监测食品产品，特别是在食品加工设施中。
