National Reference Laboratory of Veterinary Drug Residues (HZAU)/MOA Key Laboratory of Food Safety Evaluation, Huazhong Agricultural University, Wuhan 430070, China.
National Reference Laboratory of Veterinary Drug Residues (HZAU)/MOA Key Laboratory of Food Safety Evaluation, Huazhong Agricultural University, Wuhan 430070, China.
Food Chem. 2014 May 1;150:500-6. doi: 10.1016/j.foodchem.2013.11.013. Epub 2013 Nov 13.
A quantitative LC-MS/MS method was developed for the determination of chloramphenicol (CAP), thiamphenicol (TAP), florfenicol (FF) and florfenicol amine (FFA) in shrimp and fish. This rapid simple and effective extraction method was based on matrix solid-phase dispersion (MSPD). The best results were obtained using C18 as dispersant sorbent. The correlation coefficient (r) with each matrix-matched calibration curve is higher than 0.999 at the range of 0.05-0.8μg/kg for CAP and FF, 0.1-1.6μg/kg for FFA and TAP. CCα and CCβ of the fenicols upon the method were ranged from 0.01 to 0.09μg/kg and 0.04 to 0.25μg/kg respectively. In the fortified levels recoveries of the four compounds ranged from 83.8% to 98.8% with RSDs lower than 13.7%. The proposed method has been applied successfully to the analysis of CAP, TAP, FF and FFA in shrimp and fish samples, which demonstrates that this method is fast, sensitive, reliable and environmental friendly.
建立了一种用于虾和鱼中氯霉素（CAP）、甲砜霉素（TAP）、氟苯尼考（FF）和氟苯尼考胺（FFA）定量测定的 LC-MS/MS 方法。这种快速、简单和有效的提取方法基于基质固相分散（MSPD）。使用 C18 作为分散剂吸附剂可获得最佳结果。对于 CAP 和 FF，在 0.05-0.8μg/kg 的范围内，对于 FFA 和 TAP，在 0.1-1.6μg/kg 的范围内，每个基质匹配校准曲线的相关系数（r）均高于 0.999。该方法对芬考类的 CCα 和 CCβ 范围分别为 0.01-0.09μg/kg 和 0.04-0.25μg/kg。四种化合物在加标水平的回收率在 83.8%-98.8%之间，相对标准偏差（RSD）低于 13.7%。该方法已成功应用于虾和鱼样品中 CAP、TAP、FF 和 FFA 的分析，证明该方法快速、灵敏、可靠且环保。
