State Key Laboratory of Transducer Technology, Institute of Electronics Chinese Academy of Sciences, Beijing 100190, China; University of Chinese Academy of Sciences, Beijing 100190, China.
State Key Laboratory of Transducer Technology, Institute of Electronics Chinese Academy of Sciences, Beijing 100190, China.
Biosens Bioelectron. 2014 May 15;55:66-71. doi: 10.1016/j.bios.2013.11.048. Epub 2013 Nov 24.
The mammalian central nervous system (CNS) relies on a constant supply of external glucose for its undisturbed operation. This article presents an implantable Multi-Electrode Array (MEA) probe for brain glucose measurement. The MEA was implemented on Silicon-On-Insulator (SOI) wafer using Micro-Electro-Mechanical-Systems (MEMS) methods. There were 16 platinum recording sites on the probe and enzyme glucose oxidase (GOx) was immobilized on them. The glucose sensitivity of the MEA probe was as high as 489 µA mM(-1) cm(-2). 1,3-Phenylenediamine (mPD) was electropolymerized onto the Pt recording surfaces to prevent larger molecules such as ascorbic acid (AA), 3,4-dihydroxyphenylacetic acid (DOPAC), serotonin (5-HT), and dopamine (DA) from reaching the recording sites surface. The MEA probe was implanted in the anesthetized rat striatum and responded to glucose levels which were altered by intraperitoneal injection of glucose and insulin. After the in vivo experiment, the MEA probe still kept sensitivity to glucose, these suggested that the MEA probe was reliable for glucose monitoring in brain extracellular fluid (ECF).
哺乳动物中枢神经系统（CNS）依赖于外部葡萄糖的持续供应来维持其正常运作。本文提出了一种用于脑葡萄糖测量的植入式多电极阵列（MEA）探头。MEA 采用微机电系统（MEMS）方法在绝缘体上硅（SOI）晶圆上实现。探头上有 16 个铂记录位点，并且固定了酶葡萄糖氧化酶（GOx）。MEA 探头的葡萄糖灵敏度高达 489 µA mM(-1) cm(-2)。1,3-苯二胺（mPD）被电聚合到 Pt 记录表面上，以防止更大的分子，如抗坏血酸（AA）、3,4-二羟基苯乙酸（DOPAC）、血清素（5-HT）和多巴胺（DA）到达记录位点表面。MEA 探头被植入麻醉大鼠纹状体，并对通过腹腔注射葡萄糖和胰岛素改变的葡萄糖水平做出反应。在体内实验之后，MEA 探头仍然保持对葡萄糖的敏感性，这表明 MEA 探头可用于监测脑细胞外液（ECF）中的葡萄糖。
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