Iwasaki S, Tanaka H, Arima M
Radiat Res. 1987 Apr;110(1):72-83.
Wistar rats were X-irradiated in utero with 100 or 200 R on Day 13 of gestation. X Irradiation resulted in decreases not only in cerebral weight up to 15 days old but also in DNA content from Day 19 of gestation to 5 days old, and in a tendency to increase the ratio of protein to DNA in the perinatal period. The DNA contents of the homogenate, isolated nuclei, and chromatin of the cerebrum in the irradiated group were significantly lower than those in the control group. The ratio of protein to DNA at the nuclei, chromatin, and isolated DNA steps increased on irradiation. The total nucleoside content of isolated DNA determined by high-performance liquid chromatography was higher in the irradiated group than that in the control group on Day 21 of gestation but not on Day 19 of gestation. No new peaks were observed and no change in the guanine-cytosine content was seen on irradiation. X Irradiation resulted in decreases in the cytosine and deoxycytidine contents and an increase in the deoxyadenosine content. The formation of DNA-protein crosslinks in the cerebral chromatin as determined by a filter binding assay tended to increase in the irradiated groups.
在妊娠第13天，对Wistar大鼠进行子宫内X射线照射，剂量为100或200伦琴。X射线照射不仅导致15日龄以内脑重量下降，还导致从妊娠第19天到5日龄DNA含量下降，并在围产期有蛋白质与DNA比例增加的趋势。照射组大脑匀浆、分离细胞核和染色质的DNA含量显著低于对照组。照射后，细胞核、染色质和分离DNA步骤中蛋白质与DNA的比例增加。通过高效液相色谱法测定，照射组在妊娠第21天分离DNA的总核苷含量高于对照组，但在妊娠第19天则不然。照射后未观察到新峰，鸟嘌呤 - 胞嘧啶含量也未见变化。X射线照射导致胞嘧啶和脱氧胞苷含量下降，脱氧腺苷含量增加。通过滤膜结合试验测定，照射组大脑染色质中DNA - 蛋白质交联的形成有增加趋势。
