Center for Advanced Studies in Tropical Natural Resources, NRU-KU, Kasetsart University, Chatuchak, Bangkok, Thailand.
Department of Microbiology, Faculty of Science, Kasetsart University, Bangkok, Thailand.
Int J Syst Evol Microbiol. 2014 Apr;64(Pt 4):1077-1082. doi: 10.1099/ijs.0.057885-0. Epub 2013 Dec 20.
An endophytic actinomycete, strain R10-82(T), isolated from surface-sterilized roots of rice (Oryza sativa L.) was studied using a polyphasic approach. Strain R10-82(T) produced branching substrate mycelia and developed spherical spore vesicles on aerial hyphae containing non-motile spores. The major cellular fatty acids were iso-C16 : 0, iso-C14 : 0 and 10-methyl C17 : 0. The predominant menaquinones were MK-9, MK-9(H2), MK-9(H4) and MK-9(H6). Rhamnose, ribose, madurose, mannose and glucose were detected in whole-cell hydrolysates. The diagnostic phospholipids were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylinositol mannosides, hydroxylphosphatidylethanolamine and ninhydrin-positive phosphoglycolipids. These morphological and chemotaxonomic data were similar to those of the genus Sphaerisporangium. Analysis of the 16S rRNA gene sequence revealed that strain R10-82(T) was related most closely to Sphaerisporangium cinnabarinum JCM 3291(T) (98.3 % similarity). The DNA G+C content of strain R10-82(T) was 74 mol%. DNA-DNA relatedness data in combination with differences in the biochemical and physiological properties suggested that strain R10-82(T) should be classified as representing a novel species of the genus Sphaerisporangium, for which the name Sphaerisporangium rufum is proposed. The type strain is R10-82(T) ( = BCC 51287(T) = NBRC 109079(T)). An emended description of the genus Sphaerisporangium is also provided.
一株内生放线菌，R10-82(T) 株，从水稻（Oryza sativa L.）表面消毒的根部分离得到，通过多相研究进行了研究。R10-82(T) 株产生分支基质菌丝，并在含有非运动孢子的气生菌丝上发育出球形孢子囊。主要细胞脂肪酸为 iso-C16:0、iso-C14:0 和 10-甲基 C17:0。主要的menaquinones 为 MK-9、MK-9(H2)、MK-9(H4) 和 MK-9(H6)。全细胞水解物中检测到鼠李糖、核糖、甘露糖、甘露糖和葡萄糖。诊断磷脂为磷脂酰乙醇胺、双磷脂酰甘油、磷脂酰肌醇甘露糖苷、羟基磷脂酰乙醇胺和 ninhydrin 阳性磷酸甘油酯。这些形态学和化学分类学数据与 Sphaerisporangium 属相似。16S rRNA 基因序列分析表明，R10-82(T) 株与 Sphaerisporangium cinnabarinum JCM 3291(T)（98.3%相似）最为密切相关。R10-82(T) 株的 DNA G+C 含量为 74 mol%。DNA-DNA 同源性数据结合生化和生理特性的差异表明，R10-82(T) 株应归类为代表 Sphaerisporangium 属的一个新种，建议命名为红孢囊菌（Sphaerisporangium rufum）。该模式株为 R10-82(T)（=BCC 51287(T)=NBRC 109079(T)）。还提供了对 Sphaerisporangium 属的修订描述。
