Niemhom Nantawan, Chutrakul Chanikul, Suriyachadkun Chanwit, Thawai Chitti
Department of Biology, Faculty of Science, King Mongkut's Institute of Technology Ladkrabang, Bangkok 10520, Thailand.
Bioassay Laboratory, Bioresources Technology Unit, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), 113 Thailand Science Park, Phahonyothin Road, Khlong Nueng, Khlong Luang, Pathum Thani 12120, Thailand.
Int J Syst Evol Microbiol. 2017 Aug;67(8):2879-2884. doi: 10.1099/ijsem.0.002045.
A novel endophytic actinomycete, designated strain SC1-1T, was isolated from sterilized stem tissue from Stahlianthus campanulatus collected in Udon Thani province, Thailand. The isolate formed short chains of spores on aerial mycelium and presented meso-diaminopimelic acid in the cell wall peptidoglycan. Glucose, madurose, mannose, rhamnose and ribose were observed as sugars in the cells. The cell membrane phospholipids were phosphatidylethanolamine, phosphatidylmethylethanolamine, hydroxy-phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannoside and ninhydrin-positive phosphoglycolipids. The major menaquinones were MK-9(H4) and MK-9(H2). The main cellular fatty acids were iso-C16:0, 10-methyl C17 : 0 and C17 : 1ω6c. A high G+C content (70.7 mol%) was present in the genomic DNA. The taxonomic position based on the 16S rRNA gene sequence analysis revealed that strain SC1-1T belonged to the genus Nonomuraea and shared the highest 16S rRNA gene sequence similarity value with Nonomuraea dietziae DSM 44320T (98.82 %), followed by Nonomuraea africana IFO 14745T (98.58 %), Nonomuraea jabiensis A4036T (98.43 %), Nonomuraea endophytica YIM 65601T (98.36 %), Nonomuraea purpurea 1SM4-01T (98.34 %), Nonomuraea angiospora IFO 13155T (98.29 %), Nonomuraea roseola IFO 14685T (98.23 %) and Nonomuraea recticatena IFO 14525T (98.23 %). On the basis of the DNA-DNA hybridization relatedness and including the physiological and biochemical characteristics, strain SC1-1T should be judged as a novel species of the genus Nonomuraea, for which the name Nonomuraea stahlianthi sp. nov. is proposed. The type strain is strain SC1-1T (=BCC 66361T=NBRC 110006T).
从泰国乌隆他尼府采集的钟花蜘蛛抱蛋（Stahlianthus campanulatus）无菌茎组织中分离出一株新型内生放线菌，命名为菌株SC1-1T。该菌株在气生菌丝体上形成短链孢子，细胞壁肽聚糖中含有内消旋二氨基庚二酸。细胞内观察到葡萄糖、马杜拉糖、甘露糖、鼠李糖和核糖为糖类。细胞膜磷脂为磷脂酰乙醇胺、磷脂酰甲基乙醇胺、羟基磷脂酰乙醇胺、二磷脂酰甘油、磷脂酰肌醇、磷脂酰肌醇甘露糖苷和茚三酮阳性磷酸糖脂。主要的甲基萘醌为MK-9(H4)和MK-9(H2)。主要的细胞脂肪酸为异-C16:0、10-甲基-C17 : 0和C17 : 1ω6c。基因组DNA的G+C含量较高（70.7 mol%）。基于16S rRNA基因序列分析的分类地位表明，菌株SC1-1T属于野野村氏菌属（Nonomuraea），与迪茨野野村氏菌（Nonomuraea dietziae）DSM 44320T的16S rRNA基因序列相似性最高（98.82 %），其次是非洲野野村氏菌（Nonomuraea africana）IFO 14745T（98.58 %）、贾比野野村氏菌（Nonomuraea jabiensis）A4036T（98.43 %）、内生野野村氏菌（Nonomuraea endophytica）YIM 65601T（98.36 %）、紫色野野村氏菌（Nonomuraea purpurea）1SM4-01T（98.34 %）、被子野野村氏菌（Nonomuraea angiospora）IFO 13155T（98.29 %）、玫瑰色野野村氏菌（Nonomuraea roseola）IFO 14685T（98.23 %）和直链野野村氏菌（Nonomuraea recticatena）IFO 14525T（98.23 %）。基于DNA-DNA杂交相关性并结合生理生化特征，菌株SC1-1T应被判定为野野村氏菌属的一个新物种，为此提出新名称钟花蜘蛛抱蛋野野村氏菌（Nonomuraea stahlianthi）。模式菌株为菌株SC1-1T（=BCC 66361T=NBRC 110006T）。
