Salmon H, Licence S T, Binns R M
Vet Immunol Immunopathol. 1987 Feb;14(2):173-9. doi: 10.1016/0165-2427(87)90051-1.
The use of alpha-naphthyl acetate esterase (ANAE) as a T cell marker in some other species and the broad correlation of incidence of ANAE-positivity and E rosette-formation in the pig suggest that ANAE-staining may be a T-cell marker in the pig. However, by studying the staining of lymphocytes within a variety of rosettes in fixed preparations a similar incidence of pig blood lymphocytes were found to be ANAE+ among T cells (E rosettes formed in dextran), B cells (antiglobulin rosettes) and Fc-gamma receptor-bearing B and T cells (EA rosettes in saline and dextran): complement (C') receptor-bearing cells showed a higher incidence of staining than other lymphocytes. Analysis of staining morphology suggested that certain morphologies within the B and T lineages may be confined to subpopulations. Thus ANAE positivity is certainly not a marker identifying blood T lymphocytes but could be of some value indicating subpopulations of B and T lymphocytes.
在其他一些物种中，使用α-萘乙酸酯酶（ANAE）作为T细胞标志物，并且猪中ANAE阳性发生率与E花环形成之间存在广泛相关性，这表明ANAE染色可能是猪的T细胞标志物。然而，通过研究固定制剂中各种花环内淋巴细胞的染色情况，发现在T细胞（在右旋糖酐中形成的E花环）、B细胞（抗球蛋白花环）以及带有Fc-γ受体的B细胞和T细胞（在盐水和右旋糖酐中的EA花环）中，猪血液淋巴细胞中ANAE阳性的发生率相似：带有补体（C'）受体的细胞染色发生率高于其他淋巴细胞。染色形态分析表明，B细胞和T细胞谱系中的某些形态可能局限于亚群。因此，ANAE阳性肯定不是识别血液T淋巴细胞的标志物，但可能在指示B淋巴细胞和T淋巴细胞亚群方面具有一定价值。
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