Yang T J, Jantzen P A, Williams L F
Immunology. 1979 Sep;38(1):85-93.
Non-specific acid alpha-naphthyl acetate esterase (ANAE) activity was demonstrated in a majority of bovine peripheral blood lymphocytes, confirming and extending the observations on murine and human lymphocytes made by previous workers. Simultaneous study of both ANAE activity and spontaneous erythrocyte (E) or erythrocyte-antibody-complement (EAC) rosetting capability of the same bovine lymphocytes showed directly that, while 64.2 +/- 4.6 (SEM) % of bovine lymphocytes capable of forming E rosettes were ANAE positive, 38.3 +/- 0.8% of those forming EAC rosettes were also ANAE positive. Similar studies of human peripheral blood lymphocytes showed also that, while 80.6 +/- 2.2% of the lymphocytes capable of forming E rosettes were ANAE positive, 44.1 +/- 2.6% of EAC forming lymphocytes were ANAE positive. Thus the presence of ANAE activity in a majority of T lymphocytes and a significant proportion of B lymphocytes of both human and bovine peripheral blood is indicated. Human and bovine lymphocytes from phytohaemagglutinin (PHA)-stimulated cultures demonstrated greatly enhanced intensity of ANAE activity.
在大多数牛外周血淋巴细胞中证实了非特异性酸性α-萘乙酸酯酶（ANAE）活性，这证实并扩展了先前研究者对小鼠和人类淋巴细胞的观察结果。对同一批牛淋巴细胞的ANAE活性与自发红细胞（E）或红细胞-抗体-补体（EAC）花环形成能力的同时研究直接表明，能够形成E花环的牛淋巴细胞中有64.2±4.6（SEM）%为ANAE阳性，而形成EAC花环的淋巴细胞中有38.3±0.8%也为ANAE阳性。对人类外周血淋巴细胞的类似研究也表明，能够形成E花环的淋巴细胞中有80.6±2.2%为ANAE阳性，形成EAC的淋巴细胞中有44.1±2.6%为ANAE阳性。因此表明人和牛外周血的大多数T淋巴细胞以及相当比例的B淋巴细胞中存在ANAE活性。来自植物血凝素（PHA）刺激培养物的人和牛淋巴细胞显示出ANAE活性强度大大增强。
