Department of Chemistry, Massachusetts Institute of Technology , Cambridge, Massachusetts 02139, United States.
J Am Chem Soc. 2014 Jan 8;136(1):116-8. doi: 10.1021/ja411742c. Epub 2013 Dec 23.
The effect of the novel and potent monofunctional platinum(II) agent phenanthriplatin on Escherichia coli and bacteriophage λ lysogens is reported. E. coli filamentation was observed by light microscopy when cells were grown in the presence of phenanthriplatin, cis-Pt(NH3)2(Am)Cl where Am is phenanthridine. Treatment of lysogenic bacteria with this compound resulted in lysis and the production of viral particles, as indicated by plaque formation in a bacterial lawn. The results obtained with phenanthriplatin are contextualized by comparison with those obtained using cisplatin as well as other, less active, monofunctional compounds such as Pt(NH3)3Cl and cis-Pt(NH3)2(py)Cl, where py is pyridine. The ability of phenanthriplatin to induce bacterial filamentation and initiate lysis in lysogenic bacteria corroborates the hypothesis that the biological activity of this complex is mediated by its interaction with DNA.
报道了新型强效单功能铂（II）配合物菲咯嗪铂对大肠杆菌和噬菌体 λ溶源菌的影响。当细胞在菲咯嗪铂、顺式-Pt(NH3)2(Am)Cl（其中 Am 是菲咯啉）存在下生长时，通过光镜观察到大肠杆菌出现丝状化。用该化合物处理溶源菌导致裂解和病毒颗粒的产生，如在细菌菌苔上形成噬菌斑所示。用菲咯嗪铂获得的结果通过与顺铂以及其他活性较低的单功能化合物（如Pt(NH3)3Cl和顺式-Pt(NH3)2(py)Cl，其中 py 是吡啶）的结果进行比较来进行上下文化。菲咯嗪铂诱导细菌丝状化和溶源菌裂解的能力证实了这样一种假设，即该配合物的生物学活性是通过其与 DNA 的相互作用介导的。
