Szymańska-Czerwińska Monika, Galińska Elżbieta Monika, Niemczuk Krzysztof, Zasępa Magdalena
Laboratory of Serological Diagnosis/Cattle and Sheep Disease, National Veterinary Research Institute, Pulawy, Poland.
Department of Allergology and Environmental Hazards, Institute of Rural Health, Lublin, Poland.
Ann Agric Environ Med. 2013;20(4):699-704.
Coxiella burnetii is an aetiologic agent of Q fever. Ticks have been considered as a reservoir and a vector of C. burnetii. The outbreaks of Q fever were noted in different parts of Europe both in animals and humans. Q fever is zoonotic diseases characterized by sudden fever, headache, atypical pneumonia and abortions.
The aim of the study was to investigate the presence of C. burnetii infection in foresters in the south-eastern endemic region of Poland and comparison of diagnostic methods.
The serum samples were collected from 304 humans (foresters) from the 12 superintendency in the south-eastern Poland. Additionally, 1,200 ticks Ixodes ricinus were collected from the same area. The sera samples were tested by using serological methods (CFT, ELISA and IFA) and the ticks were tested with the use of real-time PCR.
The results demonstrates that infections with C. burnetii are present in foresters in the south-eastern Poland. The serological results showed both chronic and acute form of Q fever. The statistical analysis of the positive results obtained by three different serological methods revealed that values of χ2 were statistically significant between IFA and ELISA and CFT and IFA. The final percentage of examined foresters classified as positive was 10.52%. No significant differences were observed between CFT and ELISA (p=0.25).The percentage of infected ticks was 15.9%.
The study demonstrates that infections with C. burnetii are present in foresters in the south-eastern Poland. The comparison of serological methods showed that their diagnostic potential is differentiated but the most useful tool is IFA.
伯纳特立克次体是Q热的病原体。蜱被认为是伯纳特立克次体的储存宿主和传播媒介。欧洲不同地区均有动物和人类Q热疫情的报道。Q热是一种人畜共患病，其特征为突发发热、头痛、非典型肺炎和流产。
本研究旨在调查波兰东南部流行地区林业工人中伯纳特立克次体感染情况，并比较诊断方法。
从波兰东南部12个林管区的304名人类（林业工人）采集血清样本。此外，从同一地区采集1200只蓖麻硬蜱。血清样本采用血清学方法（补体结合试验、酶联免疫吸附测定和间接荧光抗体试验）检测，蜱则采用实时聚合酶链反应检测。
结果表明波兰东南部林业工人存在伯纳特立克次体感染。血清学结果显示有Q热的慢性和急性形式。对三种不同血清学方法获得的阳性结果进行统计分析发现，间接荧光抗体试验与酶联免疫吸附测定以及补体结合试验与间接荧光抗体试验之间的χ2值具有统计学意义。被归类为阳性的受检林业工人的最终百分比为10.52%。补体结合试验和酶联免疫吸附测定之间未观察到显著差异（p=0.25）。感染蜱的百分比为15.9%。
该研究表明波兰东南部林业工人存在伯纳特立克次体感染。血清学方法的比较表明它们的诊断潜力各不相同，但最有用的工具是间接荧光抗体试验。
