从质粒转化细胞中纯化大肠杆菌DNA解旋酶I
Purification of Escherichia coli DNA helicase I from plasmid-transformed cells.
作者信息
Benz I, Müller H, Abdel-Monem M, Hoffmann-Berling H
出版信息
Acta Microbiol Pol. 1986;35(3-4):191-7.
PMID:2436446
Abstract
DNA helicase I was purified in large quantity from Escherichia coli cells harboring a plasmid that carries the gene encoding helicase I--the traI gene of the F sex factor--cloned in a high copy number vector. Electron microscopic studies on the purified material reveal new properties of the enzyme protein.
摘要
从携带一个质粒的大肠杆菌细胞中大量纯化了DNA解旋酶I，该质粒带有编码解旋酶I的基因——F性因子的traI基因——克隆于一个高拷贝数载体中。对纯化物质的电子显微镜研究揭示了该酶蛋白的新特性。
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