Zhang Xing, Knochenmuss Richard, Siems William F, Liu Wenjie, Graf Stephan, Hill Herbert H
Department of Chemistry, Washington State University , Pullman, Washington 99164, United States.
Anal Chem. 2014 Feb 4;86(3):1661-70. doi: 10.1021/ac403435p. Epub 2014 Jan 14.
Ion mobility mass spectrometry (IMMS) has gained popularity in the analysis of complex mixtures such as those encountered in metabolomics and proteomics. However, the challenge that exists in conventional pulsed IMMS is its inherent low duty cycle. The first application of Hadamard transform (HT)-type signal coupled with atmospheric pressure IMMS to complex mixtures is presented. Performance of the prototype was assessed by the analysis of metabolite standard mixture. With 200 times increased IMS duty cycle in HT mode compared with conventional pulsed mode, the limit of detection (LOD) was decreased by ∼10 times. Evaluation for application to complex mixtures was achieved using the NIST Standard Reference Material 1950 Metabolites in Human Plasma. Approximately 180 metabolite ions were detected within 1 min with an IMS resolving power (Rp) of ∼100. Rapid chromatographic separation prior to IMMS analysis was also demonstrated for improving the response of metabolite ions in rat brain tissue extract.
离子淌度质谱（IMMS）在分析复杂混合物（如代谢组学和蛋白质组学中遇到的混合物）方面越来越受欢迎。然而，传统脉冲IMMS存在的挑战是其固有的低占空比。本文介绍了哈达玛变换（HT）型信号与大气压IMMS耦合在复杂混合物分析中的首次应用。通过分析代谢物标准混合物评估了该原型的性能。与传统脉冲模式相比，HT模式下的IMS占空比提高了200倍，检测限（LOD）降低了约10倍。使用美国国家标准与技术研究院（NIST）标准参考物质1950人血浆中的代谢物对复杂混合物的应用进行了评估。在1分钟内检测到约180个代谢物离子，IMS分辨率（Rp）约为100。还展示了在IMMS分析之前进行快速色谱分离，以改善大鼠脑组织提取物中代谢物离子的响应。
