College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, China; Department of Chemistry, Washington State University, Pullman, WA 99164-4630, USA.
Department of Chemistry, Washington State University, Pullman, WA 99164-4630, USA.
Food Chem. 2015 Jun 15;177:225-32. doi: 10.1016/j.foodchem.2015.01.034. Epub 2015 Jan 10.
The use of Hadamard transform ion mobility mass spectrometry (HT-IMMS) in the profiling of anthocyanins from different fruits is presented. Samples extracted with acidic methanol and purified with solid phase extraction were analyzed with direct IMMS infusion. The separation of various anthocyanins was achieved within 30s with resolving powers up to 110. The ion mobility drift times correlated with their mass-to-charge ratios with a correlation coefficient of 0.979 to produce a trend line that was characteristic for anthocyanins. Isomers with the same anthocyanidin but different hexoses were differentiated by ion mobility spectrometry. Furthermore, mobility separated ions underwent collision induced dissociation at the IMMS interface to provide MS/MS spectra. These fragmentation spectra aided in the identification of anthocyanidins via the loss of the saccharide groups. IMMS appears to be a rapid and efficient approach for profiling and identifying anthocyanins.
本文介绍了使用阿达玛变换离子淌度质谱（HT-IMMS）分析不同水果中花色苷的方法。采用酸性甲醇提取、固相萃取净化后，直接进行 IMMS 注入分析。在 30 秒内实现了各种花色苷的分离，分辨率高达 110。离子淌度漂移时间与质荷比相关，相关系数为 0.979，产生了花色苷特征性的趋势线。具有相同花色苷但不同己糖的异构体通过离子淌度谱得到区分。此外，在 IMMS 界面处，经离子淌度分离的离子发生碰撞诱导解离，提供 MS/MS 谱。这些碎裂谱通过糖基的丢失有助于花色苷的鉴定。IMMS 似乎是一种快速高效的花色苷分析和鉴定方法。
