Schander Artur, Padro David, King Hollis H, Downey H Fred, Hodge Lisa M
1 Department of Molecular Biology and Immunology, University of North Texas Health Science Center , Fort Worth, Texas.
Lymphat Res Biol. 2013 Dec;11(4):219-26. doi: 10.1089/lrb.2012.0021.
Osteopathic practitioners utilize manual therapies called lymphatic pump techniques (LPT) to treat edema and infectious diseases. While previous studies examined the effect of a single LPT treatment on the lymphatic system, the effect of repeated applications of LPT on lymphatic output and immunity has not been investigated. Therefore, the purpose of this study was to measure the effects of repeated LPT on lymphatic flow, lymph leukocyte numbers, and inflammatory mediator concentrations in thoracic duct lymph (TDL).
The thoracic ducts of five mongrel dogs were cannulated, and lymph samples were collected during pre-LPT, 4 min of LPT, and 2 hours post-LPT. A second LPT (LPT-2) was applied after a 2 hour rest period. TDL flow was measured, and TDL were analyzed for the concentration of leukocytes and inflammatory mediators. Both LPT treatments significantly increased TDL flow, leukocyte count, total leukocyte flux, and the flux of interleukin-8 (IL-8), keratinocyte-derived chemoattractant (KC), nitrite (NO2(-)), and superoxide dismutase (SOD). The concentration of IL-6 increased in lymph over time in all experimental groups; therefore, it was not LPT dependent.
Clinically, it can be inferred that LPT at a rate of 1 pump per sec for a total of 4 min can be applied every 2 h, thus providing scientific rationale for the use of LPT to repeatedly enhance the lymphatic and immune system.
整骨疗法从业者使用称为淋巴泵技术（LPT）的手动疗法来治疗水肿和传染病。虽然先前的研究考察了单次LPT治疗对淋巴系统的影响，但LPT重复应用对淋巴输出和免疫的影响尚未得到研究。因此，本研究的目的是测量LPT重复应用对胸导管淋巴（TDL）中淋巴流动、淋巴白细胞数量和炎症介质浓度的影响。
对5只杂种犬的胸导管进行插管，并在LPT前、LPT 4分钟和LPT后2小时收集淋巴样本。在休息2小时后进行第二次LPT（LPT-2）。测量TDL流量，并分析TDL中白细胞和炎症介质的浓度。两次LPT治疗均显著增加了TDL流量、白细胞计数、总白细胞通量以及白细胞介素-8（IL-8）、角质形成细胞衍生趋化因子（KC）、亚硝酸盐（NO2(-)）和超氧化物歧化酶（SOD）的通量。在所有实验组中，淋巴中IL-6的浓度随时间增加；因此，它不依赖于LPT。
临床上，可以推断每2小时以每秒1次泵的速率进行4分钟的LPT治疗，从而为使用LPT重复增强淋巴和免疫系统提供科学依据。
