Department of Molecular Biology and Immunology, University of North Texas Health Science Center, 3500 Camp Bowie Boulevard, Fort Worth, TX 76107, USA.
Exp Biol Med (Maywood). 2012 Jan;237(1):58-63. doi: 10.1258/ebm.2011.011220. Epub 2011 Dec 14.
Lymph stasis can result in edema and the accumulation of particulate matter, exudates, toxins and bacteria in tissue interstitial fluid, leading to inflammation, impaired immune cell trafficking, tissue hypoxia, tissue fibrosis and a variety of diseases. Previously, we demonstrated that osteopathic lymphatic pump techniques (LPTs) significantly increased thoracic and intestinal duct lymph flow. The purpose of this study was to determine if LPT would mobilize inflammatory mediators into the lymphatic circulation. Under anesthesia, thoracic or intestinal lymph of dogs was collected at resting (pre-LPT), during four minutes of LPT, and for 10 min following LPT (post-LPT), and the lymphatic concentrations of interleukin-2 (IL-2), IL-4, IL-6, IL-10, interferon-γ, tissue necrosis factor α, monocyte chemotactic protein-1 (MCP-1), keratinocyte chemoattractant, superoxide dismutase (SOD) and nitrotyrosine (NT) were measured. LPT significantly increased MCP-1 concentrations in thoracic duct lymph. Further, LPT increased both thoracic and intestinal duct lymph flux of cytokines and chemokines as compared with their respective pre-LPT flux. In addition, LPT increased lymphatic flux of SOD and NT. Ten minutes following cessation of LPT, thoracic and intestinal lymph flux of cytokines, chemokines, NT and SOD were similar to pre-LPT, demonstrating that their flux was transient and a response to LPT. This re-distribution of inflammatory mediators during LPT may provide scientific rationale for the clinical use of LPT to enhance immunity and treat infection.
淋巴淤滞可导致水肿和组织间液中颗粒物质、渗出物、毒素和细菌的积聚，从而导致炎症、免疫细胞迁移受损、组织缺氧、组织纤维化和多种疾病。先前，我们已经证实，整骨淋巴泵技术（LPT）可显著增加胸导管和肠导管的淋巴流量。本研究旨在确定 LPT 是否会将炎症介质转移到淋巴循环中。在麻醉下，收集犬的胸导管或肠淋巴液，在 LPT 前（pre-LPT）、LPT 期间（during LPT）和 LPT 后 10 分钟（post-LPT）测量白细胞介素-2 (IL-2)、IL-4、IL-6、IL-10、干扰素-γ、肿瘤坏死因子-α、单核细胞趋化蛋白-1 (MCP-1)、角质形成细胞趋化因子、超氧化物歧化酶 (SOD) 和硝基酪氨酸 (NT) 在淋巴中的浓度。LPT 显著增加了胸导管淋巴液中 MCP-1 的浓度。此外，与各自的 pre-LPT 流量相比，LPT 增加了胸导管和肠导管中细胞因子和趋化因子的淋巴流量。停止 LPT 10 分钟后，胸导管和肠导管中细胞因子、趋化因子、NT 和 SOD 的淋巴流量与 pre-LPT 相似，表明它们的流量是短暂的，是对 LPT 的反应。LPT 期间这些炎症介质的再分布可能为 LPT 用于增强免疫力和治疗感染的临床应用提供科学依据。
